胶原酶组细胞有一定程度损伤；
结果获得了高产量、高比活性的胶原酶。
Results The purified high producing collagenase was isolated.
目的分离获得高产量、高比活性的胶原酶。
Objective To isolate and purify collagenase with high quantity and activity.
采用酶谱方法检测IV型胶原酶对底物的降解。
Substrate degradation by type IV collagenase was measured by zymography.
肝细胞通过毛地黄皂苷-胶原酶灌流技术分离。
Hepatocytes were isolated by the digitonin-collagenase perfusion technique.
目的：研究退变颈椎间盘中的胶原酶的活性变化。
Objectives:To study collagenase activity in degenerated cervical discs.
实验趾和对照趾腱鞘内分别注入胶原酶和生理盐水。
The experimental groups were injected collagenase under sheaths, and the control groups were injected physiological saline respectively.
目的探讨胶原酶对部分椎间盘突出病人治疗无效的原因。
Objective To discuss why the Collagenase cure the intervertebral disc disease but not effective .
目的探讨胶原酶溶解术治疗腰椎间盘突出症的应用价值。
Objective To explore the applied value of collagenolysis for the treatment of lumbar disc herniation.
目的探讨胶原酶对脱矿后的人牙根面牙本质胶原蛋白的降解作用。
Objective the purpose of this study was to evaluate the effect of degradation of human root dentin collagen treated with collagenase after demineralization.
目的：探讨康复护理对胶原酶溶解术治疗腰椎间盘突出症的疗效。
Objective: To explore the effects of nursing interference on the injection of collagenase in the treatment of lumbar intervertebral disc protrusion.
目的：探讨靶位注射胶原酶治疗腰椎间盘突出术后复发的临床意义。
Objective:To investigate the clinical significance of the target-injection of collagenase for the treatment of recurrent postoperative lumbar disc herniation.
目的探讨CT引导联合靶注射胶原酶定点髓核溶解术的临床应用价值。
Objective To study the clinical significance of the combined target-injection of collagenase by ct guided for local chemonucleolysis.
目的观察兔角膜碱烧伤后形态学变化与泪液中胶原酶含量变化间的关系。
Objective To observe the relationship between morphological mutation of rabbit cornea after cornea alkali burn and the variation of collagenase content in lacrima.
也抑制内源性内皮素转化酶、胶原酶和微生物来源的金属内蛋白酶活性。
Inhibits also the activity of Endothelin Converting Enzyme, collagenase and metallo-endoproteinases from many microorganisms. Does not inhibit serine, cysteine and aspartic proteases.
目的改进侧隐窝穿刺的体表定点，筛选胶原酶注射的适应证，提高疗效。
Objective To improve the puncture point to lateral recess and select the indication of collagenase injection for lumber intervertebral disc.
有几种酶——如基质金属蛋白酶和胶原酶——可能派上用场，但矫枉过正也会有风险。
Several enzymes, such as matrix metalloproteinases and collagenases, might do the job, but overdoing it could be risky.
目的系统研究I型胶原酶-肝素诱导的大鼠脑出血后血液流变学变化情况。
Objective To investigate systematical changes of hemorheology on the model of cerebral hemorrhage induced by type I collagenase and heparin in rats.
目的：探讨CT介入靶位注射胶原酶治疗腰椎间盘突出症的临床应用价值。
Objective: To evaluate the clinical efficacy of the target-injection of collagenase under ct intervention for the treatment of lumbar disc herniation.
目的：观察胶原酶化学溶解术治疗颈椎间盘突出症的远期疗效及影响因素。
Objective: to investigate the influential factors and clinical therapeutic effect of epidural injection of collagenase for the treatment of cervical intervertebral disc protrusion.
目的观察间质胶原酶(MMP - 1)表达质粒对大鼠肝纤维化的影响。
Objective To observe tile effects of MMP-1 (Matrix Metalloproteinase-1) expressing plasmid on rat liver fibrosis.
目的研究臭氧髓核氧化术结合胶原酶溶解术治疗腰椎间盘突出症的临床疗效。
Objective To assess the therapeutic effect of oxygen-ozone combined collagenase injection for the treatment of lumbar disc herniation.
胶原酶是一种特异降解胶原螺旋区或明胶，而不作用于其它蛋白质底物的酶类。
Collagenase is one kind of enzymes that can degrade native helical collagen fibrils or glutin but not degrade others proteins.
目的探讨CT导引下硬膜外穿刺置管注射胶原酶治疗颈椎间盘突出症的临床疗效。
Objective To investigate the clinical effects of injecting collagenase by epidural puncture tube under ct guidance for the treatment of cervical intervertebral disc prolapse.
结论：靶位注射胶原酶溶盘术可与手术互补，适用于腰椎间盘突出术后复发的治疗。
Conclusion:The target-injection of collagenase for intervertebral disk chemolysis may be complementary therapy for recurrent postoperative lumbar disc herniation.
逆转录多聚酶链反应检测间质胶原酶及金属蛋白酶组织抑制因子- 1基因表达水平。
The gene expression levels for interstitial collagenase and tissue inhibitor of metallo-proteinase-1 (TIMP-1) were measured by reverse transcription polymerase chain reaction.
前言：目的：研究CT引导下胶原酶注射颈椎间盘溶解术治疗颈椎间盘突出症的价值。
Objective: To study the values of treating the cervical disc herniation by collagenase under ct guiding.
目的研究经皮穿刺腰椎间盘切除术和胶原酶化学溶解术联合治疗腰椎间盘突出症的疗效。
Objective to investigate the therapeutic effect of percutaneous lumbar diskectomy (PLD) combining with chemonucleolysis (CNL) by injection of collagenase in the treatment of lumbar disc herniation.
以18天鸡胚睾丸为实验材料，经胶原酶和胰蛋白酶两步酶消化法得到生精上皮细胞悬液。
Testes from 18-day-old embryonic chickens were used to isolate seminiferous epithelial cells by sequential two-step enzyme digestion with collagenase and trypsin.
以18天鸡胚睾丸为实验材料，经胶原酶和胰蛋白酶两步酶消化法得到生精上皮细胞悬液。
Testes from 18-day-old embryonic chickens were used to isolate seminiferous epithelial cells by sequential two-step enzyme digestion with collagenase and trypsin.
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