其技术主要包括胚胎活检、聚合酶链反应及荧光原位杂交。
The basic techniques currently used involve embryo biopsy, the polymerase chain reaction and fluorescence in situ hybridization.
方法采用胚胎期至老年期小鼠全胚胎及脑切片的原位杂交染色。
Methods Embryonic to aged stage murine embryo and brain sections were stained by whole-mount in situ hybridization technique.
蛋白酶K处理条件是否适当是小鼠全胚胎RNA原位杂交成功的关键因素。
The key factor of the success to mouse whole mount RNA in situ hybridization is whether the proteinase K treatment conditions are optimal or not.
接下来利用爪蟾胚胎致畸实验（FETAX）、组织切片技术和整体原位杂交技术，研究了三种酰胺类除草剂对热带爪蟾胚胎的致畸效应。
Next, using FETAX test, tissue section and whole mount in situ hybridization technique to study the teratogenic effect of three herbicides on early stages of development of X. tropicalis.
接下来利用爪蟾胚胎致畸实验（FETAX）、组织切片技术和整体原位杂交技术，研究了三种酰胺类除草剂对热带爪蟾胚胎的致畸效应。
Next, using FETAX test, tissue section and whole mount in situ hybridization technique to study the teratogenic effect of three herbicides on early stages of development of X. tropicalis.
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