体外培养观察软骨细胞贴壁和生长均较缓慢。
The in vitro observation showed that cell adherence and growth was slow.
提取的骨髓基质细胞呈贴壁生长,诱导后可向成骨细胞分化,细胞呈成骨细胞形态为梭形和多角形。
BMSC grew by static adherence, differentiated to osteoblasts and had strong capacity of proliferation, the shape of cells was fusiform or polygon, like osteoblasts.
结果表明:各组细胞正常贴壁,生长良好,细胞形态正常。
The results showed: each group of cells grown well and the form of cell was normal.
进一步通过离心、差速贴壁和化学试剂抑制非心肌细胞生长,纯化得到95%以上的心肌细胞。
By centrifugal selection, differential paces of sticking to wall and medical inhibit method, purification of cardiac myocytes reach to 95 %.
结果大鼠msc为均一的梭形的成纤维细胞样生长,贴壁及增殖能力强,生长曲线呈s型。
The rat MSC were uniform spindle-shaped in appearance and showed active proliferative capacity and had s shape of growth curve.
复苏后分离所得胎肝细胞用锥虫兰染色评估细胞活率,并记录贴壁时间,观察集落生长情况。
The survival rate of thawed cells was evaluated by trypan blue staining, and the adherence time and colony growth were observed.
对贴壁细胞的生长及增殖活性进行观察;
Then growth and proliferation of the cells were observed from the micrograph.
结论视网膜细胞混合培养有利于神经节细胞的贴壁和生长。
Culturing retinal cells in commixture benefits the paste and growth of ganglion cells.
结果丝素膜能支持细胞在其表面贴壁,生长曲线和染色体正常。
Results Bone marrow stem cells can attach and grow on silk fibroin films; growth curve and chromosome were normal.
观察不同分离条件对表皮细胞生长情况、贴壁率、克隆形成率的影响。
The output of viable cells, rates of adherence to dish, colony forming efficiency(CFE) of the isolated epidermal ce.
该细胞贴壁生长,具有接触抑制的性质。
These cells were able to grow adhering to the wall and stop growing with contact inhibition.
细胞呈贴壁生长,趋向复层,无接触抑制。
The cell line grew with adherence, showing a tendency of multi layer growth without contact inhibition.
结果大鼠骨髓基质细胞贴壁呈集落生长,5 -氮胞苷诱导骨髓基质细胞转化为心肌样细胞。
Results Mesenchymal stem cells were spindle-shaped and active proliferation capacity in vitro, they can be differentiation into cardiac-like muscle cells after adding 5-azacytidine.
结果:丝素蛋白膜上生长的HS G细胞数量最多,贴壁良好,形态为多边形,细胞突起多而长。
Results: the number of HSG cells on SF membranes was more than that on the other materials, the cells appeared polygonal shape and attached well with long processes and more microvilli.
利用生物化学和细胞培养方法对研制出的新型羧甲基壳多糖微载体CX-2的生物亲和性、细胞贴壁及生长和扩展特性进行了研究。
The properties of the newly developed microcarrier (MC) CX-2 were investigated using biochemical and cytological techniques, including bio-affinity, cyto-compatibility, eurytopicity, and so on.
结果丝素膜能支持细胞在其表面贴壁,生长曲线和染色体正常。
The effects of attachment, growth curve and cytogenetic property of bone marrow stem cells on silk fibroin films were studied by viable cell counts and detection of chromosome aberration rate.
利用淋巴细胞分离液分离出单核细胞,采用贴壁生长法分离纯化内皮祖细胞。
Lymphocyte separation medium was used to isolate the mononuclear cells, and the attachment growth method was used to purify endothelial progenitor cells.
DOTAP复合物的转染效率明显高于游离AS-ODN;细胞是否贴壁生长及贴壁生长的细胞密度也影响AS-ODN的转染效率。
AS-ODN/DOTAP complex could exhibited more higher transfection efficiency than free AS-ODN and the transfection efficiency was also influenced by many other factors such as cell density etc.
结果与结论:胎盘间充质干细胞、骨髓间充质干细胞均成贴壁生长、形态均一的成纤维样细胞梭形外观,但后者体积略小。
RESULTS AND CONCLUSION:PMSCs and BMSCs had the adherent growth ability, morphology of fibroblast-like cells and spindle-shaped appearance, but the latter had the slightly smaller size.
方法使用密度梯度离心法分离不同年龄段脂肪间充质干细胞进行培养,保留贴壁细胞传代,分析脂肪间充质干细胞的纯度,观察细胞生长情况,检测其增殖活性、细胞周期。
The adherent cells were preserved to passage, the purity of ADSCs was analyzed by immunocytochemistry method, and cell growth was observed, then proliferation capability and cell cycle were detected.
方法使用密度梯度离心法分离不同年龄段脂肪间充质干细胞进行培养,保留贴壁细胞传代,分析脂肪间充质干细胞的纯度,观察细胞生长情况,检测其增殖活性、细胞周期。
The adherent cells were preserved to passage, the purity of ADSCs was analyzed by immunocytochemistry method, and cell growth was observed, then proliferation capability and cell cycle were detected.
应用推荐