微泡浓度为10％时细胞转染效率最高。
Microbubbles at concentration of 10% led to the highest transfection effect.
方法：用磷酸钙共沉淀的方法向血管成纤维细胞转染MKP1的基因；
Methods: MKP 1 gene was transfected into vascular fibroblasts by the classical calcium phosphate coprecipitation technique.
目的：考察不同脂质体材料对阳离子脂质体细胞转染率和脂质体膜流动性的影响。
Objective: To investigate the influences of different lipids on cellular delivery and fluidity of liposome membrane of cationic liposome.
本文就神经干细胞、TRAIL及神经干细胞转染TRAIL后与胶质瘤的关系作一综述。
This article will review the relationship among NSCs, TRAIL a nd glioma cells.
结论采用绿色荧光蛋白为报告基因的真核细胞转染技术中，脂质体法是效率高、安全性大的方法。
ConclusionUsing GFP as a report gene in eukaryotic cell transfection, the lipofectamine method could get a higher transfection efficiency and higher survival rate for cells.
已有实验表明，细胞转染效率可能决定于DNA -脂质体复合物的形成以及所转染的细胞种类。
Previous studies have shown that the level of gene expression achieved may be dependent on the formulation of the DNA-liposome complex and the cell type transfected.
结果：卵巢癌细胞转染反义ERCC1基因后，ERCC1基因转录水平、蛋白表达水平明显下降。
Results: After transfection of the antisense ERCC1, Northern and Western blotting indicated that the RNA and protein expression level of ERCC1 was obviously decreased.
目的考察并确定多聚乙烯亚胺（PEI）最佳的体外细胞转染条件，评价PEI作为基因转染试剂的可行性。
OBJECTIVE To investigate the optimum condition of polyethylenimine(PEI) for gene transfer to A549 cells.
最近的研究表明脂质体在转染呼吸道上皮细胞中是有用的。
Recent studies have shown lipoplexes to be useful in transfecting respiratory epithelial cells.
该抗血清不但能识别来源于大肠杆菌的抗原，还能检测真核细胞内转染后表达的IRF-7。
The antisera can recognize not only recombinant IRF-7 expressed in E. coli, but also transfected IRF-7 in mammalian cells.
含有叶酸的聚合载体能够识别肿瘤表面受体，并且能够通过受体介导的(细胞)内摄作用转染细胞。
Polymeric carrier containing folic acid is expected to be able to identify tumor surface receptors and transfect cells by receptor-mediated endocytosis.
使用该抗体证实，转染293t细胞后表达的IRF - 7主要分布在细胞质内。
We also showed that the IRF-7 was localized mainly in the cytoplasm of transfected 293t cells.
MTT法显示转染组的细胞增殖力低于未转染组和转染空载体组（P<0.05）。
The MTT showed cell proliferation ability was lower in the transfection group than in the non-transfection and mock-vehicle groups (P<0.05).
目的：以CHO - K1细胞为宿主基因瞬时转染条件的优化。
Objective: To optimize the condition of gene transient transfection in CHO-K1 cell.
目的研究阳离子脂质体膜的组分对细胞摄取和基因转染的影响。
OBJECTIVE To study the effect of the lipid compositions on cellular uptake and gene transfection mediated by cationic liposomes.
目的：在中国仓鼠卵巢(CHO)细胞上稳定转染咪唑啉1受体。
Objective: to stably transfect imidazoline-1 receptor (I 1r) in Chinese hamster ovary (CHO) cells.
目的以电穿孔法转染COS7细胞系以得到较高的转染率。
Objective To optimize the transfection rate in COS 7 cell line with electroporation method.
摘要腺病毒载体具有高效转染和低细胞毒性的特点，已成为基因治疗研究中应用最广泛的载体之一。
Adenovirus vectors have the characteristics of high transfection efficiency and low cytotoxicity, and they have become most widely used vectors for gene therapy.
分别用细胞和夹心酶联免疫吸附试验（ELISA）检测DNA转染细胞膜表面抗原。
The membrane surface antigens of DNA-transfected cells were detected with cellular ELISA and sandwich ELISA respectively.
抗原，CD59；糖尿病；突变；cho细胞；转染；抗补体活性。
Antigens, CD59; Mutation; CHO cells; Transfection; Anticomplement activity.
所观察的几种肿瘤中的微血管内皮细胞孔窗及内皮细胞裂隙与转染的目的基因无明显的关系，且内皮细胞的凋亡均不明显。
No correlation between gene transfection and fenestra formation or cleft of capillary endothelial cells was observed, and no apoptosis of endothelial cells was found.
在标准转染条件下，增加DNA用量和延长细胞暴露时间反而使转染效率下降。
Under standard transfection conditions, increasing DNA amount and exposure time of the DNA-liposome complexes with cells resulted in decreased transgene expression.
利用基因转染方法使细胞获得不死性，快速建立小鼠胸腺基质细胞（MTSC）系。
For rapid establishment of mouse thymic stromal cell(MTSC) lines, gene transfection was used to immortalize fresh viable cells.
目的：构建人死亡受体5 (DR5)真核表达载体，转染NS - 1细胞，建立稳定转染的NS - 1细胞系。
Objective: to construct eukaryotic expression vector of human death receptor (DR5) and transfect NS-1 cells to establish stable NS-1 cell line.
目的以RNA依赖性腺嘌呤脱氨酶（ADAR1）为靶基因优化悬浮培养的小鼠原代淋巴细胞的电穿孔转染条件。
Objective To optimize the electroporation parameters in mouse primary lymphocyte cultured in suspension with adenosine deaminase acting on RNA 1(ADAR1) as a reporter gene.
目的探讨转染表皮细胞生长因子(EGF)基因的人表皮细胞移植后egf的表达及对细胞增殖的影响。
Objective to investigate the epithelial growth factor (EGF) expression of EGF gene-transfected keratinocytes and its effect on cell proliferation after grafting.
LMP可明显抑制DMSO对CNE1细胞的诱导分化作用，转染细胞诱导后增殖力和角蛋白表达无显著改变（P＞0．05）。
The differentiation of CNE1 cells induced by DMSO was inhibited by LMP, there were no changes on the proliferation and keratin expression after induction of cells transfected by LMP gene ( P >0 05).
结果合成的DNA转运系统和经修饰的病毒载体体外转染靶细胞后，可获得显著表达。
Results Artificial synthetic DNA transfer system and modified viral vectors could efficiently transfect target cells and get high level expression.
方法细胞瞬时和稳定转染及氯霉素酰基转移酶和芳香族化酶活性测定。
Methods Transient and stable transfection experiments in cells and assays of chloramphenicol acetyltransferase activity and aromatase activity were used.
方法细胞瞬时和稳定转染及氯霉素酰基转移酶和芳香族化酶活性测定。
Methods Transient and stable transfection experiments in cells and assays of chloramphenicol acetyltransferase activity and aromatase activity were used.
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