用端粒重复序列扩增法(TRAP)半定量方法测定细胞染毒前后端粒酶活性的变化;
The changes of telomerase activity after treatment was detected and quantified by telomeric repeat amplification protocol (TRAP) semi_quantitative analysis.
目的探讨经香烟烟雾溶液染毒的人正常肺间质细胞和人肺腺癌细胞的DNA损伤及其修复效应。
Objective to study the DNA damage and repair of normal lung interstitial cells and human lung adenocarcinoma cells exposed to cigarette smoke.
各染毒组细胞DNA链断裂的迁移长度显著增加,且存在剂量效应关系。
The movements of DNA migration in the BALF and lung cells increased with a dose effect relationship.
结果随苯染毒浓度的增加,染毒时间的延长,骨髓细胞DNA迁移率和迁移度增加。
Results DNA damage of bone marrow cells increased with the increase of benzene concentration and time of treatment.
目的研究HSP70在乐果染毒u 87细胞中的保护作用。
Objective To investigate the protective effect of HSP70 in U87 cell line treated with dimethoate.
目的探讨二硫化碳染毒对小鼠心肌细胞凋亡的影响。
Objective to study the effect of carbon disulfide infection of mice myocardial cell apoptosis.
结果:随染毒剂量的增加,染料木素对L 5178y的细胞毒性增大。
Results: Cytotoxicity of genistein for L5178Y cells increased dose-dependently.
从细胞和整体水平都显示铅染毒组巨噬细胞吞噬功能明显降低。
The phagocytosis of macrophages of lead-treated significantly decreased both at cellular and whole animal levels.
结果各水样处理H4IIE细胞后,H4IIE细胞的增殖随染毒时间和剂量的增加而呈下降趋势。
Result After exposed to the extracts, the proliferation of H4IIE cell decreased according to the exposure time and dose.
结果染毒后小鼠脾、肝、肺、肾组织细胞DNA出现损伤,且损伤程度与染毒剂量具有一定相关性。
Results DNA of spleen, liver, lung and kidney cells was significantly damaged after exposure, and there were some correlation between exposure concentration and damage degree.
MTT法测定氰戊菊酯对MLTC-1细胞活力的影响,确定染毒剂量。
The cell viability of MLTC-1 cells affected by fenvalerate was analyzed using the method of MTT. 3.
目的应用牛胸腺dna和染毒丙烯腈(AN)小鼠精细胞研究an对DNA的交联作用,研究AN毒作用的分子机制。
Objective to study the cross-link effect on DNA and the toxic mechanism of acrylonitrile (an) using calf thymus DNA and spermatids of an exposed mice.
目的观察砷、氟及砷氟联合染毒对SGC 790 1细胞基因组dna的损伤作用及特点。
Objective To study the features of DNA damage induced by arsenic, fluoride and their combination in cultured SGC 7901 cells.
方法:通过建立新生大鼠皮层神经元原代培养,用不同浓度醋酸铅染毒和给予牛磺酸干预,观察神经细胞的生长和存活情况。
Methods:Put taurine and different concentrations of lead acetate in cultures of cortex neurons and observe the length of dendites and the growth activity of neurons.
采用染色体畸变试验,检测染毒万蛾灵后小鼠骨髓淋巴细胞染色体畸变率的改变。
Before and after the mice were contaminated with various dosage of Wanelin, the rate of chromosome aberration of marrow lymphocytes were tested by chromosome aberration assay.
目的利用三氯乙烯(TCE)染毒的小鼠模型,研究T、B淋巴细胞及细胞因子在TCE诱发过敏反应中的作用。
Objective To investigate the effects of T and B lymphocytes and cytokines on allergic reaction induced by trichloroethylene(TCE)in mice.
目的研究甲醛和苯联合染毒对小鼠脾脏、胸腺淋巴细胞DNA和RNA合成的影响。
Objective To explore the effect of combined exposure of formaldehyde and benzene on DNA and RNA synthesis in spleen and thymic lymphocytes in mice.
结论亚急性BHT大剂量染毒可造成大鼠肺、肝等脏器功能性和器质性损伤,BHT可诱发肺巨噬细胞凋亡的产生。
Conclusion BHT may cause functional and organic damage of lung and liver, and it can induce apoptosis to pulmonary macrophage in rats.
结果:随着染毒时间延长,腰髓前角神经细胞膜上的嵌入蛋白质由分布紊乱至成簇聚集。
Results: as the extending of inhaling time inserting proteins distribution changed from turbulence to cluster.
随染毒时间延长,同一染毒剂量组内细胞增殖活性变化率有下降趋势,有一定染毒时间-效应关系。
Cell proliferation rate declines with the prolongation of exposure time to DMF, and time-effect relationship exists.
随着TOCP染毒时间的延长,细胞内mda和CAT含量均呈明显的时间—效应关系。
With the TOCP treatment time prolonged, intracellular MDA and CAT levels showed obvious time-effect relationship.
高剂量染毒组动物主动脉弓、心肌、肝和大脑皮质透射电镜观察结果显示:主动脉弓内皮细胞出现肿胀、细胞核变形、部分核染色质溶解、染色质边聚现象;
The endothelial cells of aortic arch were swelling, the nuclei of which was twisted, a part of chromatin was dissolved and migrated to the cell edge.
染毒后细胞突起结构逐渐消失,细胞体积变小、形态变圆。
After the treatment of rotenone cell ecptomas vanished gradually and cell bodies became smaller and smoother.
采用流式细胞术检测,G2/M期细胞百分数增加,1/50LD50染毒组时与对照组比较差异具有显著性(P<0.05)。
The percentage of cells at G2/M phase was increased with the increasing of MeHg, and the 1/50 LD50 group had significant difference compared with control group (P<0.05).
目的研究HSP70在乐果染毒U87细胞中的保护作用。
[Objective] To investigate the protective effect of HSP70 in U87 cell line treated with dimethoate.
目的]观察孕鼠香烟烟雾染毒致胎鼠眼畸形的作用及对视杯、视泡上皮细胞和间充质细胞凋亡的影响。
Objective To study the affection of the hyperthmia and cigarette smoking on the neonate rats neurobehavioral.
目的]观察孕鼠香烟烟雾染毒致胎鼠眼畸形的作用及对视杯、视泡上皮细胞和间充质细胞凋亡的影响。
Objective To study the affection of the hyperthmia and cigarette smoking on the neonate rats neurobehavioral.
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