方法用酶消化、分离鸡胚关节软骨细胞，体外单层培养，收集细胞培养液。
Methods Chick embryo joint chondrocytes digested and isolated by enzyme were grown monolayer culture in vitro. Chondrocyte culture media was collected.
材料和方法：采用酶辅助的显微分离法分离新生牛眼ipe细胞，酶消化法分离角膜内皮细胞，进行体外细胞培养。
Material and Methods: From bovine eyes IPE cells were isolated with enzyme-assisted microdissection, corneal endothelium cells with enzyme digestion and then were cultured in vitro.
方法采用体外细胞培养技术进行皮肤成纤维细胞的分离、培养、传代、冻存、复苏及鉴定。
Methods Cell culture in vitro technique was used, including isolation, culture, subculture, frozen storage, recovery and identification of human fibroblasts, in this study.
方法采用体外细胞培养技术进行皮肤成纤维细胞的分离、培养、传代、冻存、复苏及鉴定。
Methods Cell culture in vitro technique was used, including isolation, culture, subculture, frozen storage, recovery and identification of human fibroblasts, in this study.
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