方法应用免疫组织化学方法检测erk在脊髓后角的定位表达，并用图像分析系统和免疫印迹法进行定量分析。
Methods The expression of ERK in spinal dorsal horn was detected by immunohistochemical method and was quantitated by western-blot analyses.
方法：用免疫印迹法检测aif在细胞中的表达量；用免疫组化法检测aif在29例胃癌组织和10例癌旁正常胃组织中的表达。
Immunohistochemical method was used to detect the expression of AIF in 29 cases of gastric cancer tissues and 10 cases of normal gastric mucosa.
方法采用免疫组织化学技术、图像分析、放射免疫和免疫印迹法。
Methods Immunohistochemical technique, image analysis, radioimmunoassay and Western blotting were used.
核酸杂交试验：是检测HPV感染的重要的手段，包括斑点印迹法（dotblothybridization）、组织原位杂交法、核酸印记法（Southern blot hybridization）。
Nucleic acid hybridization test: it is an important means to detect HPV infection, including dot blot method (blot hybridization dot), tissue in situ hybridization, blot hybridization (Southern).
方法 采用免疫组织化学染色法、免疫印迹法，分析检测食管癌标本中EZH2蛋白的表达。
We then detected EZH2 protein expression in the esophageal squamous carcinoma, paracarcinomatous and normal tissues by Western blotting.
新的抗体连接方法是为蛋白质印迹法专门设计的。后者是一种凝胶电泳法可根据细胞和组织样本的分子重量对其进行分离。
The new antibody conjugates are designed for Western blotting applications, which use gel electrophoresis to separate proteins from a sample of cell or tissue based on molecular weight and size.
采用免疫组化方法、半定量逆转录聚合酶链反应（RT-PCR）和免疫印迹分析法检测COX-2、CDKN2A在组织中的表达。
The expression of COX-2 and CDKN2A was determined by immunohistochemistry, semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot.
采用免疫组化方法、半定量逆转录聚合酶链反应（RT-PCR）和免疫印迹分析法检测COX-2、CDKN2A在组织中的表达。
The expression of COX-2 and CDKN2A was determined by immunohistochemistry, semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot.
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