用端粒重复序列扩增法(TRAP)半定量方法测定细胞染毒前后端粒酶活性的变化;
The changes of telomerase activity after treatment was detected and quantified by telomeric repeat amplification protocol (TRAP) semi_quantitative analysis.
方法取19例颅内肿瘤和5例脑外伤患者脑脊液标本,应用改良的银染端粒重复序列扩增法(TRAP)进行端粒酶活性检测。
Methods a modified telomeric repeat amplification protocal (TRAP) by silver staining was performed in 19 cases of intracranial tumors and 5 cases of brain injuries.
方法采用端粒重复序列扩增法一酶联免疫(PCR-TRAP)检测70例肺癌手术患者癌组织中的端粒酶活性,并与70例肺部良性疾病患者对照组的检测结果进行比较。
Methods Use PCR-TRAP detection of 70 cases of lung cancer patients' telomerase activity in cancer tissue. and 70 patients with benign lung disease and the results were compared.
方法采用端粒重复序列扩增文件-酶标法(TRAP-ELISA)测定尖锐湿疣皮损中端粒酶的活性,并与正常皮肤和恶性肿瘤对照比较。
Methods The telomerase activity was detected by TRAP( telomeric repeat amplification protocol) -ELISA which based upon PCR amplification of the initial telomerase product and detected by ELISA.
方法:采用端粒重复序列扩增文件酶标法(TRAP-ELISA),对32例AML和非恶性血液系统疾病骨髓细胞端粒酶活性检测。
Methods:The telomerase activity was determined in bone marrow cells from 32 patients of acute myeloid leukemia and non-malignant hematogic disease by TRAP-ELISA.
方法:采用端粒重复序列扩增文件酶标法(TRAP-ELISA),对32例AML和非恶性血液系统疾病骨髓细胞端粒酶活性检测。
Methods:The telomerase activity was determined in bone marrow cells from 32 patients of acute myeloid leukemia and non-malignant hematogic disease by TRAP-ELISA.
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