端粒重复序列扩增酶联免疫吸附法测定端粒酶活性；
Telomerase activity was also examined by telomeric repeat amplification protocol enzyme-linked immunosorbent assay (TRAP-ELISA).
目的探讨应用银染端粒重复扩增技术(TRAP)检测乳腺癌组织端粒酶活性。
Objective to detect telomerase activity in breast carcinoma by silver-staining telomeric repeat amplification protocol (TRAP).
端粒酶（网站链接 ）包含一个重要的核糖核酸成分，是补充端粒重复序列。
The telomerase ( web link ) contains an essential RNA component which is complementary to the telomere repeat sequence.
用端粒重复序列扩增法（TRAP）半定量方法测定细胞染毒前后端粒酶活性的变化；
The changes of telomerase activity after treatment was detected and quantified by telomeric repeat amplification protocol (TRAP) semi_quantitative analysis.
目的：建立端粒重复序列扩增(TRAP)-银染色技术，探讨端粒酶活性检测在肿瘤诊断中的意义。
Objectives: to establish a method of telomere repeat amplification protocol (TRAP) silver stain technique, and to investigate the significance of telomerase activity detection in tumor diagnosis.
方法取19例颅内肿瘤和5例脑外伤患者脑脊液标本，应用改良的银染端粒重复序列扩增法(TRAP)进行端粒酶活性检测。
Methods a modified telomeric repeat amplification protocal (TRAP) by silver staining was performed in 19 cases of intracranial tumors and 5 cases of brain injuries.
方法P CR -ELISA以地高辛标记端粒重复片段特异的探针与PCR变性产物杂交，通过ELISA检测端粒酶活性。
Methods We used telomerase PCR-ELISA an assay in which a digoxigenin labeled telomerase repeat-specific probe is hybridized to the PCR product detecting with ELISA.
方法：采用端粒重复序列扩增文件酶标法（TRAP-ELISA），对32例AML和非恶性血液系统疾病骨髓细胞端粒酶活性检测。
Methods:The telomerase activity was determined in bone marrow cells from 32 patients of acute myeloid leukemia and non-malignant hematogic disease by TRAP-ELISA.
方法采用端粒重复序列扩增文件-酶标法（TRAP-ELISA）测定尖锐湿疣皮损中端粒酶的活性，并与正常皮肤和恶性肿瘤对照比较。
Methods The telomerase activity was detected by TRAP( telomeric repeat amplification protocol) -ELISA which based upon PCR amplification of the initial telomerase product and detected by ELISA.
方法采用端粒重复序列扩增法一酶联免疫（PCR-TRAP）检测70例肺癌手术患者癌组织中的端粒酶活性，并与70例肺部良性疾病患者对照组的检测结果进行比较。
Methods Use PCR-TRAP detection of 70 cases of lung cancer patients' telomerase activity in cancer tissue. and 70 patients with benign lung disease and the results were compared.
方法采用端粒重复序列扩增法一酶联免疫（PCR-TRAP）检测70例肺癌手术患者癌组织中的端粒酶活性，并与70例肺部良性疾病患者对照组的检测结果进行比较。
Methods Use PCR-TRAP detection of 70 cases of lung cancer patients' telomerase activity in cancer tissue. and 70 patients with benign lung disease and the results were compared.
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