以HPV-18 DNA为供体、小牛胸脉DNA 为载体，NIH3T3细胞为受体细胞，应用磷酸钙沉淀法将供体DNA 引入细胞内，得到了清晰的NIH 3T3细胞形态学转化灶。
NIH 3T3 cells were transfected by human papillomavirus 18(HPV 18). HPV 18 DNA as donor and calf thymus DNA as vector. Calcium phosphate technique was used.
本研究认为鱼精蛋白改性磷酸钙是一种比共沉淀法合成磷酸钙更好的转染载体。
It could be expected that nucleoprotamine modified calcium phosphate could be a better transfect agent as compared to coprecipitated superphosphate.
方法：人血管形成素- 1重组粘粒与DNA末端蛋白复合物混合后以磷酸钙共沉淀法转染2 93细胞。
METHODS: Angiopoietin-1-PAxCAwt cosmid DNA and adenoviral DNA-TPC were cotransfected to 293 cells by the use of calcium phosphate precipitation method.
方法：人血管形成素- 1重组粘粒与DNA末端蛋白复合物混合后以磷酸钙共沉淀法转染2 93细胞。
METHODS: Angiopoietin-1-PAxCAwt cosmid DNA and adenoviral DNA-TPC were cotransfected to 293 cells by the use of calcium phosphate precipitation method.
应用推荐