然后通过碳二亚胺法将半抗原与载体蛋白偶联制备人工抗原，采用紫外扫描及SDS - PAGE鉴定。
Then, antigen was prepared by conjugation of hapten with carrier protein by carbodiimide (EDPC) method and was identified by UV spectroscopy and SDS-PAGE.
采用优化的碳二亚胺法制备诺氟沙星(NOR)人工抗原，紫外扫描和红外光谱图表明人工抗原偶联成功。
Modified EDC method was employed to synthesize the artificial antigen of norfloxacin (nor), which was proved by Ultraviolet absorbance and IR spectra.
方法A群脑膜炎球菌多糖经溴化氰活化后共价接上己二酰肼手臂，在碳二亚胺催化下与精制破伤风类毒素（TT）偶联制备结合物。
Methods Conjugate was prepared by carbodiimide mediated coupling of adipic acid hydrazide derivatives of capsular polysaccharides of group A meningococcal with tetanus toxoid(TT).
传统的肽类合成多采用液相化学合成，常用的包括碳二亚胺法，混合酸酐法、酰氯法、叠氮物法等等，为多肽的合成提供了多种选择。
Peptides are traditionally synthesized in liquid phase, such methods are DCC, mixed anhydrides, acyl chloride and azide etc. , which provide more choices for peptides synthesis.
首次研究了碳二亚胺14与手性氨基酸酯的成环反应，结果表明：在室温下，碳二亚胺14与手性氨基酸酯能快速成环生成一个五员环。
The cyclization reactions of carbodiimide 14 with chiral amide ethyl ester was firstly studied. The results showed that the carbodiimide 14 reacted quickly with amide ethyl ester at room temperature.
方法T-2毒素与琥珀酸酐在吡啶参与下于蒸汽浴中反应，生成T- 2 - 半琥珀酸酐后，再与小牛血清白蛋白经碳二亚胺偶联结合生成T- 2毒素抗原。
Methods T 2 toxin in pyridine reacted with succinic anhydride in a steam bath and converted to T 2HS. At the presence of EDPC, T 2HS conjugated with BSA, then BSA T 2HS was prepared.
以二环己基碳化二亚胺为活化剂将葡萄糖氧化酶(GOD)共价键接在玻碳电极上。
Glucose oxidase (GOD) was attached to glassy carbon electrodes by covalent binding with dicyclohexylcarbodiimide as an activator.
以二环己基碳化二亚胺为活化剂将葡萄糖氧化酶(GOD)共价键接在玻碳电极上。
Glucose oxidase (GOD) was attached to glassy carbon electrodes by covalent binding with dicyclohexylcarbodiimide as an activator.
应用推荐