本研究旨在构建具有猪 MSTN 前肽基因定点突变的真核表达载体。
This research intended to construct a eukaryotic expression vector with a site-directed mutation of porcine MSTN propeptide gene.
“我本来以为他在开玩笑，直到他开始往下走才信以为真。”肯尼迪事后一边说，一边在一排电视摄像机前出示那张假条。
"I thought he was joking until he started walking down," Kennedy said after the event, showing off the note in front of a bank of television cameras.
我儿子坐在电视机前看英超的比赛，不知道他是怎么学会打开卫星电视频道的，真讨厌。
My son sits in front of the television watching English Premier League soccer. Somehow he has discovered how to unblock satellite TV. I hate that.
DNA证据显示，真兽类和有袋类最后的共同祖先生活在1.6亿年前左右。
DNA evidence has suggested the last common ancestor to eutherians and marsupials lived around 160 million years ago.
在发现Juramaia前，已知的最早的真兽类动物是生活在1.25亿年前的Eomaia。
Before Juramaia's discovery, the oldest known eutherian was Eomaia that lived 125 million years ago.
乡村音乐和排名前四十的音乐的爱好者们，于其它风格的音乐的爱好真们相比，更趋向于是传统型的，诚实的，保守的人。
Fans of country and Top 40 hits tend to be more conventional, honest and conservative compared with fans of other genres.
26岁的法尔范也是所效力俱乐部沙尔克04的真核所在，上个赛季，沙尔克能够在欧冠赛场上取得前四强的历史性成绩与他的表现是分不开的。 法尔范是在周五的训练中受伤的，他需要接受进一步的扫描检查以确定伤势。
The 26-year-old, who inspired his German club side Schalke 04 to the semi-finals of last season's UEFA Champions League, sustained a thigh injury in training on Friday.
真该死，我必须在制服短路前赶快到维修站去。
Damn it, I have to get to the service office before my suit short-circuits.
你敢在牧师前这样开玩笑，真了不起。
Oh! You are a one, telling that joke in front of the priest.
当时我悟出最大的幸福不是真把一块太妃糖送进嘴里，而是吃前对它目不转睛的凝望。
And when I discovered that the greatest pITch happiness was not in actually eating a toffee but in gazing at IT beforehand.
真漂亮啊，她想着，在公寓前停了下来。
一位母亲说：“我真着急。女儿都奔三了，她工作很忙，交往圈子又小。我特别希望她能在28岁前结婚，30岁之前生孩子。”
She has a busy job and a limited number of acquaintances. I really hope she can get married before 28 and have a baby before 30.
我前一个女朋友真喜欢人家注意她。如果你不注意她，她就会想办法让你注意她。
My last girlfriend was an attention junkie! If you weren't looking at her, she would do something to draw attention to her.
可是，即使我们晓得真理，在熟谙真道前，我们仍然会表现笨拙，毫无优美可言。
And yet even when we know the truth, we can still be awkward and graceless at times until we get the feel of it.
本研究旨在克隆通城猪含有第1个内含子的MSTN前肽基因，构建真核定点诱变载体，并通过转染C2C12细胞验证载体表达的有效性。
This research intended to construct eukaryotic expression vector with a site-directed mutation of porcine MSTN propeptide gene, and verify its expression efficacy in C2C12 cells.
真高兴在你走前能有机会和你谈谈，玛丽。祝你一路平安。
I'm really glad I got a chance to talk to you before you left, Mary. Have a safe trip!
武当山各主要宫殿均建有龙虎殿，供奉青龙白虎，以渲染朝拜真武的临前威仪。
Mount Wudang major palaces were built with dragon and tiger Temple, where landmarks of white tiger to Zhenwu exaggerated worship of the Provisional authority when the former.
那是5年前，她在出国临行时，送给我这个名为“幸运之船”的礼物，它周身是银白色，并且夹杂着一些漂亮的花纹，十分像一个真船。
It was about 5 years ago, she gave me this present before going abroad and she called it lucky boat. It is silver coloured with some pretty patterns. Everything looks exactly like a real boat.
那个抓着一名妇女挡在身前的人真拿着枪吗？
Does the man holding a woman in front of him really have a gun or not?
铁斋向浦原道歉，在他们能说其他东西前，真子突然尖叫。
Tessai apologizes to Urahara and before they can speak more Shinji suddenly scream out.
首先讨论了真镇定带前通的广义系统的最小阶正常观测-控制器的设计问题，然后基于降阶观测-控制器，明确给出了广义系统双互质分解的一种新的状态空间实现。
The design problem of minimal order observer based controllers for properly stabilizing a singular system with direct control feed through term is discussed, and a design method is given.
《狱中上母书》是少年英雄夏完淳临刑前写的家书，信中既流淌着作者至真至纯的个人情感，也表现了忠孝节义和宗族意识的民族文化心理。
The letter presented his truest personal love and hatred, and also showed ethical psychological characters of loyalty, filial piety and natural religious belief.
真声被用于在细小的片段上创造语音，该片段在被呼叫者使用前要结合在一起。
Real voices are used to create the speech in tiny fragments that are glued together before being played to the caller.
我真软弱，极其软弱，只能俯伏你脚前；
我真软弱，极其软弱，只能俯伏你脚前；
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