本研究建立了西方马脑炎病毒的RT-PCR检测方法，应用于实验感染西方马脑炎病毒的来亨鸡血液及蚊虫样本中的病毒核酸检测。
A pair of primers were synthesized, and related RT-PCR assay was established to detect the western equine encephalitis virus(WEEV)in experimentally infected mosquitoes and Leghorn chicken.
使用丙肝病毒重组免疫印迹分析技术和丙肝病毒核糖核酸检测对丙肝病毒感染进行确诊。
HCV recombinant immunoblot assay (RIBA) and HCV RNA testing are used to confirm the diagnosis of HCV infection.
目的探讨不同数量混合血清样本丙肝病毒核酸（HCVRNA）检测在拟输血手术患者中的临床应用。
Objective To investigate the clinical application of detecting hepatitis C virus ribonucleic acid (HCV RNA) using various number of serum samples from patients planning to receive blood transfusion.
聚合酶链反应(PCR)技术用于病毒核酸的检测具有灵敏度高，特异性强等优点，已广泛用于各型肝炎病毒的实验室检测。
Polymerase Chain Reaction (PCR) technique has been widely used in detecting different hepatitis viruses in laboratory, because of its high specificity and sensitivity.
方法对广西境内15种117只野生动物及99只人工养殖的果子狸采用聚合酶链反应和血清学方法检测其病毒核酸。
Methods Serological test and PCR method were used to detect 117 of 15 species of wild animals and 99 domesticated Paguma larvata.
目的建立人感染高致病性禽流感病毒H5N1的核酸检测方法，用于人感染高致病性禽流感病毒疑似病例临床标本的检测。
Objective To establish a method for H5N1 RNA detection and laboratory diagnosis of suspected human avian influenza (H5N1) virus infected cases.
结果采样后经中国疾病预防控制中心检测，H5N1禽流感病毒核酸阳性，并分离出h5n1禽流感病毒。
Results the samples were tested AIV-RNA positive by the Chinese Center for Disease Control and Prevention, and the H5N1 avian influenza virus were separated from the samples.
目前人免疫缺陷病毒感染的实验室诊断主要依靠抗体检测，其他还包括P24抗原检测、核酸检测、病毒的分离培养等辅助检测手段。
Diagnosis of human immunodeficiency virus(HIV) infection mostly rely on the detection of HIV-1/2 antibody, P24 antigen, HIV RNA, virus culturing can be used as additional test.
结果：RT-PCR可在心脏、肝脏、脾脏、肾脏以及脑组织内检测到病毒核酸，透射电镜下可在肝细胞胞浆内观察到形态典型的病毒粒子。
R esu lts:The nuc le ic ac id of CCHF virus could be detected in the hearts, livers, spleens, kidneys and brains of the infected suckling mice by the RT-PCR.
实验结果证实 LAMP技术可成功应用 于猪瘟病毒核酸序列之检测。
The results from the above experiments suggested that LAPM technique can be successfully applied in the detection of classical swine fever virus.
方法：采集流感样病例咽拭子，提取标本中的病毒核酸，用荧光定量pcr方法检测。
Method: Collected throat swab of influenza-like cases, extracted viral RNA in throat swab specimens and detected the viral RNA by FQ-PCR.
方法：采集流感样病例咽拭子，提取标本中的病毒核酸，用荧光定量pcr方法检测。
Method: Collected throat swab of influenza-like cases, extracted viral RNA in throat swab specimens and detected the viral RNA by FQ-PCR.
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