用线性化质粒DNA 电穿孔前环锥虫体，筛选其对磷霉素的抗性。
Procyclic trypanosomes were electroporated with linearised plasmid DNA and selected for resistance to phleomycin.
电脉冲；电场分布；电穿孔。
Electrical pulse; the distribution of electric field; electroporation.
温度和缓冲液中的血清成分对电穿孔效率影响不大。
The operation temperature and the concentration of serum in buffer have no effect on the transfection efficiencies.
小鼠和鸡分别经电穿孔和肌肉注射免疫两次，间隔为3周。
The mice and chickens were immunized twice, 3 weeks apart, by electroporation into muscles or intramuscular injection.
目的研究电穿孔技术对小分子离子型药物经皮渗透的影响。
OBJECTIVE To study the effects of electroporation on the permeation of small molecular ion drug through skin in vitro.
目的以电穿孔法转染COS7细胞系以得到较高的转染率。
Objective To optimize the transfection rate in COS 7 cell line with electroporation method.
分别改变脉冲电压和环境温度，研究了酵母细胞的电穿孔情况。
Separately changes the pulse voltage and temperature in the study of yeast cells by electroporation.
将正确连接的表达质粒线性化后用电穿孔法转化到毕赤酵母GS115中。
The correct plasmid was linearized and transformed into Pichia Pastoris strain GS115 by electroporation.
本文采用一种新的方法—电穿孔结合化学药物进行肿瘤治疗的电化学疗法。
In this paper, we will report a new approach for the treatment of tumor-electroporation therapy.
目的研究电穿孔及离子导入时电场方向对分子型药物咖啡因经皮渗透的影响。
Objective to study the effect of electric field direction on the percutaneous penetration of the non-ionic drug caffeine mediated by skin electroporation and iontophoresis.
方法构建含tK基因的逆转录病毒表达载体，电穿孔法转化肺癌细胞A549。
Methods a retroviral vector containing TK gene was constructed and transduced into pulmonary adenocarcinoma cell A549 by electroporation.
结果说明，利用电穿孔可提高抗肿瘤药物的细胞毒性，为治疗肿瘤提供了新的途径。
Accordingly, the electroporation can enhance the cytotoxicity of antitumor drug. This is a piece of new approach for antitumor.
本文重点阐述了电穿孔在基因治疗中的优势、影响因素及在临床前和临床试验中应用。
This article mainly elaborated the superiority of electroporation in gene therapy and the influencing factors, as well as the applications in the preclinical and clinical trial.
是指利用电穿孔和电渗透的原理，无创无痛的把活性成份或营养导入到皮下的美容疗法。
Electroporation refers to the use of electro-osmosis and the principle of non-invasive painless to import active ingredients or nutrition to beauty therapy skin.
脉冲后将老鼠放回笼子内，几分钟后老鼠恢复正常的活动，电穿孔不会对老鼠有任何的副作用。
Return mouse to cage after pulsing. Mouse will resume normal activities within minutes. EP does not cause any side effects on mouse.
本论文共分为四部分：第一部分，研究了利用电脉冲对细胞电穿孔，提高抗癌药物治疗肿瘤细胞的疗效。
In the first part, we have studied the improving drug's effect on the treatment of tumor by electric pulse.
阐述了化学导入法(PEG)、电穿孔法、注射法，粒子轰击法、花粉管萌导法及使用其它载体系统的成功实例。
The direct gene transfer systems involved the chemical method (PEG), electroporation, microinjection, particle bombardment, pollen-mediated gene transfer and other systems using various vectors.
目的以RNA依赖性腺嘌呤脱氨酶（ADAR1）为靶基因优化悬浮培养的小鼠原代淋巴细胞的电穿孔转染条件。
Objective To optimize the electroporation parameters in mouse primary lymphocyte cultured in suspension with adenosine deaminase acting on RNA 1(ADAR1) as a reporter gene.
第一部分研究了强电脉冲可导致离体鸡血细胞产生电穿孔，获得了最直接的形态学证据，并对电穿孔产生的机理作了初步探讨。
In the first section, that electroporation can be induced on chick's erythrocytes by high-intensity electric pulses was investigated. The most direct proof of morphology was acquired.
例如，ED5可以减少小鼠尿路梗阻（单侧输尿管结扎）或通过电穿孔法切除肾脏7天后皮质早期生长反应基因-1信使RNA表达和间质纤维化。
For example, ED5 reduces cortical Egr-1 mRNA expression and interstitial fibrosis in rats 7 days after renal obstruction (unilateral ureteral ligation) and delivery by electroporation.
例如，ED5可以减少小鼠尿路梗阻（单侧输尿管结扎）或通过电穿孔法切除肾脏7天后皮质早期生长反应基因-1信使RNA表达和间质纤维化。
For example, ED5 reduces cortical Egr-1 mRNA expression and interstitial fibrosis in rats 7 days after renal obstruction (unilateral ureteral ligation) and delivery by electroporation.
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