方法：运用甲基化特异性P CR，检测RASSF1A基因在48例喉癌组织、相对应癌旁组织及48例正常人外周血中的启动子区甲基化情况。
Methods a methylation-specific PCR was performed to detect the promoter methylation of RASSF1A gene in 48 tumor tissues, 48 corresponding normal tissues and 48 normal blood plasma.
目的：建立检测肝癌胰岛素样生长因子ii (IGF - II)基因启动子p3甲基化状态的方法。
Aim: to detect the methylation at the promoter P3 of human insulin-like growth factor II (IGF-II) gene in hepatocellular carcinoma.
方法采用改良MS P法检测胃癌患者胃黏膜组织中DAPK基因的甲基化情况。
Methods Methylation state of DAPK in gastric cancer was detected by this improved MSP.
非甲基化胞嘧啶残基的亚硫酸氢盐转换，接着是检测方法，例如不同位点的测序已经被接受为检测5甲基胞嘧啶的金标准。
Bisulphite conversion of unmethylated cytosine residues followed by detection methods such as sequencing of distinct loci has become accepted as the gold standard for detecting 5-methylcytosines.
方法采用RT P CR技术、硫化pcr结合限制性内切酶技术检测白血病细胞系及正常人外周血单个核细胞WT 1基因的表达及其启动子区DNA甲基化水平。
Method The expression of WT1 gene and its DNA methylation status were assayed in leukemia cell lines and normal peripheral blood mononuclear cells (PBMNC) by RT-PCR and MS-PCR.
方法：采用PCRSSCP法检测血管网织细胞瘤中VHL基因的突变率及甲基化，敏感限制性内切酶消化法检测血管网织细胞瘤中VHL基因的异常甲基化率。
Methods: The hypermethylation was examined by methyl sensitive restrictive DNA endoenzyme analysis in 34 cases of angioreticuloma and the VHL gene mutations detected by PCR SSCP analysis.
结论：本文所建立的高效液相色谱法能够准确灵敏地检测全基因组总体甲基化水平，检测结果稳定，方法重现性好；
Conclusions:The HPLC method that we have established for assaying the genome-wide DNA total methylation level is stable and reproducible;
结论：本文所建立的高效液相色谱法能够准确灵敏地检测全基因组总体甲基化水平，检测结果稳定，方法重现性好；
Conclusions:The HPLC method that we have established for assaying the genome-wide DNA total methylation level is stable and reproducible;
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