这里，我们概述了当前用于甲基化检测的试剂，及它们在微阵列和测序平台上的应用。
Here, we outline the currently used methylation detection reagents and their application to microarray and sequencing platforms.
结果：在最小限度的假阴性及假阳性结果中，TLX3和FOXE3DNA甲基化检测可有效鉴别ALL。
Results: Reliable detection of DNA methylation of TLX3 and FOXE3 segregated ALL from those clear of disease with minimal false-negative and false-positive results.
对人体DNA甲基化水平进行检测发现，结合在DNA上的甲基团数量与年龄有关，因此，唾沫分析技术有望成为犯罪现场取证工具。
An analysis of a person's DNA methylation finds that the number of methyl groups attached to the DNA correlates with the person's age — making saliva analysis a possible CSI tool.
他们的团队对一组10到19岁的儿童进行了糖皮质激素受体基因甲基化的检测，并对他们的妈妈做了同样的检测。
Their team examined the methylation of the glucocorticoid-receptor gene in a group of children ranging in age from ten to 19 years, and in those children's mothers.
目的检测皮肤基底细胞癌(BCC)中T钙黏蛋白的表达以及异常甲基化。
Objective To detect the expression and aberrant methylation of T-cadherin in cutaneous basal cell carcinoma (BCC).
目的：建立检测肝癌胰岛素样生长因子ii (IGF - II)基因启动子p3甲基化状态的方法。
Aim: to detect the methylation at the promoter P3 of human insulin-like growth factor II (IGF-II) gene in hepatocellular carcinoma.
方法：运用甲基化特异性P CR，检测RASSF1A基因在48例喉癌组织、相对应癌旁组织及48例正常人外周血中的启动子区甲基化情况。
Methods a methylation-specific PCR was performed to detect the promoter methylation of RASSF1A gene in 48 tumor tissues, 48 corresponding normal tissues and 48 normal blood plasma.
目的：评价特异性甲基化pcr (msp)分析法检测抑癌基因高甲基化对结、直肠癌的诊疗价值。
Aim: To evaluate the clinical value of MSP (methylation specific PCR) in detection of tumor suppressor genes methylation in the colorectal cancer.
方法采用改良MS P法检测胃癌患者胃黏膜组织中DAPK基因的甲基化情况。
Methods Methylation state of DAPK in gastric cancer was detected by this improved MSP.
目的建立甲基化特异性PCR（MSP）技术检测黏着斑激酶相关性鸟苷三磷酸酶调节因子（GRAF）基因启动子甲基化。
Objective To establish the methylation-specific PCR(MSP) to detect methylation of GTPase regulator associated with the focal adhesion kinase(GRAF) gene promoter.
肿瘤组织未检测到甲基化患者的血清中及健康人血清中均未检测到该基因甲基化变异。
No aberrant methylation of APC gene was found in the serum samples from healthy control and the patients without gene methylation in tumor tissue.
目的：检测BLU与ARF基因启动子在鼻NK/T细胞淋巴瘤中的甲基化状态，探讨其在肿瘤发生中的作用与分子病理诊断中的应用价值。
AIM: To investigate the hypermethylation of the BLU and ARF promoter in nasal NK/T cell lymphoma from nasopharyngeal mucosa and its roles in tumor genesis and molecular pathological diagnosis.
非甲基化胞嘧啶残基的亚硫酸氢盐转换，接着是检测方法，例如不同位点的测序已经被接受为检测5甲基胞嘧啶的金标准。
Bisulphite conversion of unmethylated cytosine residues followed by detection methods such as sequencing of distinct loci has become accepted as the gold standard for detecting 5-methylcytosines.
同时通过检测胃癌组织中基因的蛋白表达水平，了解基因高甲基化与其蛋白表达缺失之间的关系。
The expression of p16 and DAPK protein were detected to analyse the relationship between the promoter hypermethylation and the loss of protein expression.
目的检测SFRP1基因在非小细胞肺癌（NSCLC）组织和血浆中的甲基化状态，分析其临床意义。
Objective To detect the promoter methylation status of SFRP1 gene in tissue and plasma DNA of non-small cell lung cancer(NSCLC) patients and evaluate its correlation with clinicopathological features.
亚硫酸氢钠测序检测表达有差异的基因调节序列的甲基化状态。
Bisulfite sequencing was used to determine the methylation status of the regulatory sequence in which gene the expression are significantly difference.
方法：采用PCRSSCP法检测血管网织细胞瘤中VHL基因的突变率及甲基化，敏感限制性内切酶消化法检测血管网织细胞瘤中VHL基因的异常甲基化率。
Methods: The hypermethylation was examined by methyl sensitive restrictive DNA endoenzyme analysis in 34 cases of angioreticuloma and the VHL gene mutations detected by PCR SSCP analysis.
方法采用RT P CR技术、硫化pcr结合限制性内切酶技术检测白血病细胞系及正常人外周血单个核细胞WT 1基因的表达及其启动子区DNA甲基化水平。
Method The expression of WT1 gene and its DNA methylation status were assayed in leukemia cell lines and normal peripheral blood mononuclear cells (PBMNC) by RT-PCR and MS-PCR.
联合检测SFRP基因家族甲基化状态对于食管癌的预后判断有一定指导意义。
Combination analysis of methylation status in SFRP genes may has definite value on estimating prognosis of ESCC.
结论：本文所建立的高效液相色谱法能够准确灵敏地检测全基因组总体甲基化水平，检测结果稳定，方法重现性好；
Conclusions:The HPLC method that we have established for assaying the genome-wide DNA total methylation level is stable and reproducible;
结论：本文所建立的高效液相色谱法能够准确灵敏地检测全基因组总体甲基化水平，检测结果稳定，方法重现性好；
Conclusions:The HPLC method that we have established for assaying the genome-wide DNA total methylation level is stable and reproducible;
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