阳性培养物用生化反应、协同凝集试验、代谢抑制试验、聚合酶链反应和DNA序列测定等方法进行鉴定。
Biochemical reaction, coagglutination test, metabolism inhibition test, polymerase chain reaction (PCR) assay, and DNA sequencing were employed to identify the isolated microorganisms.
用逆转录-聚合酶链反应(RT - PCR)方法检测主动外排泵基因CDR1和CDR2的表达水平。
Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to measure the mRNA levels of active efflux gene CDR1 and CDR2.
采用聚合酶链反应(PCR)方法扩增rho基因第1 ~ 5外显子和第1内含子基因片段，用直接dna测序法筛查rho基因突变。
Extron 1-5 of RHO gene was amplified by polymerase chain reaction (PCR), and the mutation of RHO gene was screened by direct DNA sequence measurement.
方法用盐提取法提取人群中白细胞的DNA，以聚合酶链反应、限制性内切核酸酶检测-4533G/A多态性。
Methods DNAs were extracted from the white blood cells of people in Hainan by salt-out method. Polymerase chain reaction and restriction endonuclease was used to determine the 4533G/A polymorphism.
方法采用逆转录聚合酶链反应（RT- PCR）测定大鼠淋巴细胞CGRP m RNA水平。用放射性标记法测定血浆CGRP水平。
Method Expression of CGRP mRNA in lymphocytes was determined by the semi quantity RT PCR and the CGRP levels in plasma were measured by RIA.
方法：用甲基化敏感的限制性核酸内切酶消化，结合聚合酶链反应（PCR）技术。
Methods: 20 cases of MDS patients were studied using methylation sensitive restriction enzyme digestion and polymerase chain reaction(PCR) technique.
方法用半巢式聚合酶链反应方法检测72例非甲—庚型肝炎患者血清中ttv - DNA。
Methods TTV-DNA in serum of 72 patients with hepatitis Non-A to G were detected by Hn-PCR.
用聚合酶链反应和单链构象多态性（ PCR-S SCP）结合序列测定方法，检测了66例健康对照I L-10启动子区多态性的发生。
The Polymorphisms of IL 10 Promoter region were detected by PCR SSCP and sequencing. 66 of health control were examined by this way.
方法对116例随机收集的1型糖尿病患者用聚合酶链反应-限制性内切酶消化作该点突变的筛选；
Methods The mutation had been screened from 116 unrelated patients with type 1 diabetes mellitus by ApaI digestion of product of polymerase chain reaction amplification.
采用病例对照研究的方法，用聚合酶链反应(PCR)和限制性片段长度多态性分析(RFLP)研究TS患者与健康对照间DRD2P、COMT基因的基因型频率有无统计学差异。
DRD2P and COMT genotyping were carried out using PCR followed by RFLP and statistical analysis of genotype frequencies between ts patients and healthy controls was performed using SPSS programme.
方法采用聚合酶链反应(PCR)方法，用自行设计的特异引物，对昆虫的钠离子通道基因进行扩增。
Methods PCR method was applied to detect the KDR mutation of the target site (sodium channel) gene.
方法采用聚合酶链反应(PCR)方法，用自行设计的特异引物，对昆虫的钠离子通道基因进行扩增。
Methods PCR method was applied to detect the KDR mutation of the target site (sodium channel) gene.
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