方法：应用免疫组化法检测乳腺癌及相应癌旁组织tlr4、增殖细胞核抗原(pcna)的表达，TUNEL法检测组织中细胞的凋亡。
Methods: the expression of TLR4 and PCNA in cancer tissues and adjacent tissues were assessed by immunohistochemical method and the apoptosis was detected by the TUNEL method.
分别采用免疫组化方法和原位末端标记法检测垂体前叶细胞增殖核抗原的表达及凋亡的变化情况。
The changes of the proliferating nuclear antigen expression and apoptosis of anterior pituitary cells were detected with immunohistochemistry and in situ end labeling method.
目的：检测血管内皮生长因子(VEGF)和增殖细胞核抗原(pcna)在胶质瘤中表达，并探讨其与肿瘤恶性程度的关系。
Objective: To examine the expressions of VEGF and PCNA in gliomas, and investigate the possible relationship between their expressions and the degree of tumor malignancy.
应用免疫组化方法检测增殖细胞核抗原和P-选择素的表达。
The local expression of proliferating cell nuclear antigen(PCNA) and P-selectin were analyzed by immunohistochemical technique.
目的：检测环上颌骨骨缝区增殖细胞核抗原阳性表达，探讨缝牵张促进上颌骨生长发育的作用。
AIM To detect proliferating cell nuclear antigen PCNA positive expression in suture cells around maxilla and explore the improvement of suture expansion on the growth and development of maxilla.
免疫组化学检测增殖细胞核抗原（PCNA）。EGFR、细胞角蛋白（CK）的表达。
The expression of PCNA, EGFR and CK was assessed by immunohistochemical stains.
方法：应用SP免疫组化法，检测P 21, P27等细胞周期依赖性激酶抑制剂和增殖细胞核抗原(pcna)在角膜上皮不同部位的表达情况。
METHODS: the expressions of CKI, P27, P21 and proliferating cell nuclear antigen (PCNA) were tested in different regions of corneal epithelium by SP immunohistochemistry.
同时，检测胰腺癌增殖细胞核抗原(pcna)作为胰腺癌增殖状态的指标。
Pancreatic cancer proliferation cell nuclear antigen (PCNA) was also tested as the index of the extent of proliferation of the pancreatic cancer.
方法用流式细胞仪检测缺血缺氧后不同时间点星形胶质细胞细胞周期变化，并用荧光免疫细胞化学技术测定胶质细胞纤维酸性蛋白（GFAP）和增殖细胞核抗原（PCNA ）的表达水平。
Methods We measured the astrocyte cell cycles in different time after ischemia and anoxia by flow cytometry and detected the levels of GFAP and PCNA with fluorescence immunocytochemistry.
疗程结束后，取大鼠结肠组织，免疫组化方法检测其增殖细胞核抗原(pcna)的表达，流式细胞仪分析其细胞周期的变化。
Proliferating cell nuclear antigen (PCNA) expression of colon tissue was determined by immunohistochemical method, the changes of cell cycle was tested by flow cytometry.
疗程结束后，取大鼠结肠组织，免疫组化方法检测其增殖细胞核抗原(pcna)的表达，流式细胞仪分析其细胞周期的变化。
Proliferating cell nuclear antigen (PCNA) expression of colon tissue was determined by immunohistochemical method, the changes of cell cycle was tested by flow cytometry.
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