染色体原位杂交技术是现代生物技术的重要组成部分。
The chromosome in situ hybridization is an important part in modern biotechnology.
通过粗线期染色体荧光原位杂交技术,将发生易位的第6号和第12号染色体的易位点分别锚定在1个和3个BAC克隆库中。
Transpositional points between chromosome 6 and 12 were anchored in 1 and 3 BAC clone's pool respectively by fluorescence in situ hybridization technology of pachytene chromosomes.
目的探讨用多色荧光原位杂交(MFISH)技术检测的易位和双着丝粒染色体畸变的差异。
Objective To explore the differences between radiation-induced translocation and dicentric chromosome aberrations detected by multicolor fluorescence in situ hybridization (M-FISH) method.
结论与传统的染色体显带技术相比,荧光原位杂交技术具有高效、灵敏、可靠的特点,可为临床提供良好的辅助诊断。
Conclusions Compared with the conventional chromosome banding technique, FISH has characteristics of high sensitivity and accuracy, and provides a good assist method for diagnosis.
抗病易位系Y96060可通过细胞遗传分析,染色体C分带和原位杂交技术,RAPD技术得到确认。
Said disease-resisting translocation line Y96060 can be confirmed by means of cytogenetic analysis, chromosome C zoning and in situ hybridization and RAPD technique.
目的建立多色荧光原位杂交技术检测人卵细胞染色体非整倍体的方法。
Objective: To establish the method of aneuploidy detection in human oocytes by multicolor fluorescence in-situ hybridization (M-FISH).
方法:取试管婴儿助孕技术后未能受精成功的卵细胞,采用多色荧光原位杂交方法检测卵细胞13,16 ,18,2 1和2 2号染色体的情况。
Methods:Multicolor fluorescence in situ hybridization (M-FISH) was applied to check the chromosome status in oocytes for chromome 13,16,18,21 and 22.
方法:取试管婴儿助孕技术后未能受精成功的卵细胞,采用多色荧光原位杂交方法检测卵细胞13,16 ,18,2 1和2 2号染色体的情况。
Methods:Multicolor fluorescence in situ hybridization (M-FISH) was applied to check the chromosome status in oocytes for chromome 13,16,18,21 and 22.
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