造血干细胞体外扩增技术有望克服这一障碍。
Ex vivo expansion of cord blood might help to overcome this limitation.
方法：采用随机引物扩增技术（RAPD）对菌株进行分析。
Methods: The strains are analyzed using random amplified polymorphic DNA (RAPD).
方法：应用PCR扩增技术检测244名广东籍汉族人ace基因型。
Method: PCR was applied to determine ACE genotype from 244 Guangdong Han nationality people.
技术人员从唾液样本中提取DNA，然后采用扩增技术产生大量的DNA拷贝。
Technicians extract DNA from a sputum specimen and then use amplifying techniques to produce large Numbers of copies of the DNA.
目的探讨应用银染端粒重复扩增技术(TRAP)检测乳腺癌组织端粒酶活性。
Objective to detect telomerase activity in breast carcinoma by silver-staining telomeric repeat amplification protocol (TRAP).
本研究对环介导等温扩增技术的原理、特性及其应用做一综述，从而为其临床应用提供理论依据。
This article reviews the principles, characteristics and application of LAMP, with an aim to provide the theoretical basis of its clinical application.
本文综述了DNA序列分析、P CR限制性片段长度多态性技术和环介导等温扩增技术在带绦虫虫种鉴别中的应用。
This paper summarizes the application of molecular biological techniques in the identification of Taenia, such as analysis of DNA sequence, PCR-RFLP and LAMP.
P CR是一种体外核酸扩增技术，具有敏感性强、特异性高、快速、简便等优点，在医学、生物学等领域中得到了广泛应用。
As a technique for amplifying nucleic acid in vitro with high sensitivity, specificity, swiftness and convenience, PCR has been applied widely in the aspects of medicine and biology nowadays.
为了建立弓形虫的快速检测方法，本研究建立了一种灵敏、特异、快速的分子生物学检测方法——环介导等温扩增技术（LAMP）。
In order to develop a rapid and simple method for Toxoplasma gondii infection, a novel loop-mediated isothermal amplification(LAMP) assay for T.
利用DNA合成技术、DNA克隆技术、PCR扩增技术以及DNA芯片技术，结合密码学的计算复杂度理论，提出了一种基于DNA技术的加密方法。
Using the technologies of DNA synthesis, DNA clone, PCR and DNA chip and the theory of computational complexity, an encryption scheme is formed.
大多数分子标记体系的利用都取决于聚合酶链反应（PCR），这是一项扩增特定DNA序列的重要技术。
Use of most molecular marker systems depends on the polymerase chain reaction (PCR), which is an important technique for amplifying specific DNA sequences.
这项技术是根据提取小量遗传学样本，快速扩增大量样本进行研究。
The technique is based on taking small genetic samples and rapidly copying them to provide a large enough sample to study.
目的建立变形菌随机扩增dna多态性分型技术，应用于临床菌株流行病学调查。
OBJECTIVE To establish the random amplification of polymorphic DNA (RAPD) typing technology for Proteus strains and apply it in the epidemiology investigation.
运用RAPD技术对小麦光敏雄性不育基因进行了分子标记，并对小麦RAPD扩增体系进行了优化。
The RAPD techniques were used for molecular marker of wheat Photo -sensitive male sterile genes and RAPD amplification system was optimized.
应用甲基化敏感扩增多态性(MSAP)技术对24个脐橙品种胞嘧啶甲基化模式和程度进行评估。
The extent and pattern of cytosine methylation in 24 navel orange cultivars, genomes were assessed by using the technique of methylation-sensitive amplified polymorphism (MSAP).
目的：建立端粒重复序列扩增(TRAP)-银染色技术，探讨端粒酶活性检测在肿瘤诊断中的意义。
Objectives: to establish a method of telomere repeat amplification protocol (TRAP) silver stain technique, and to investigate the significance of telomerase activity detection in tumor diagnosis.
方法随机扩增多态性DNA技术(RAPD)。
Methods To use a random amplified polymorphic DNA technique (RAPD).
目的建立随机扩增多态性DNA技术(RAPD)对甲氧西林耐药溶血性葡萄球菌(MRSH)的分型方法。
Objective To establish the Random Amplified Polymorphic DNA (RAPD) Technique for typing methicillin resistant Staphylococcus haemolyticus (MRSH).
而高gc模板扩增作为P CR技术中的瓶颈问题之一，目前仍然没有得到较好的解决。
However, amplification of GC-rich templates, as one of the bottle necks in PCR, has not been solved thoroughly.
方法从胚胎大鼠的大脑皮质、海马、纹状体等组织分离神经干细胞，用无血清培养技术在体外进行培养、扩增、传代和诱导分化。
The advantage of serum free and clone culturing technology was performed to isolate, culture, passage and induce neural stem cells from embryonic rat cortex, hippocampus and striatum.
方法：利用无血清培养和细胞克隆培养技术，从胚胎大鼠海马、纹状体、脊髓等区分离神经干细胞，进行体外扩增培养、传代、贴壁分化观察。
Methods: The advantage of serum free and clone culturing technology was performed to isolate, culture and passage neural stem cells from embryonic rat hippocampus, striatum and spinal cord.
目的利用直接扩增片段长度多态(DALP)这一新分子标记技术建立一套稳定的红景天属植物的DALP反应体系。
Objective Direct amplification of length polymorphism (DALP) as a new molecular marker was used to establish a set of stable DALP reaction system for the plants of Rhodiola l.
提示PCR扩增DNA技术对结核性渗出性胸膜炎是高度敏感和特异的早期、快速诊断方法。
Therefore, the paper suggests PCR amplification DNA is a highly sensitive and specific method for diagnosing tuberculous exudative pleurisy early and rapidly.
在52扑克影院：惊魂记，罗德以标签扩增实境技术，重看后现代希治阁大师之作《惊魂记》，为这出划时代的惊世距著作出新的诠译。
In 52 Card Psycho, Rhodes USES marker-based augmented reality technology to look back on the late modern Hitchcock masterpiece, Psycho - which has become an icon of artistic appropriation.
建立和优化了大麦dna单限制性酶切选择性扩增多态性技术体系(SADF)。
A method named selective amplification DNA fragments (SADF) using single restrictive enzyme was established and optimized in barley.
用菌体脂肪酸分析和随机dna基因扩增分析技术进行菌株间的同源性研究。
Study of homogeneity were carried through by Thalli fatty acid profile analysis and randomly amplified polymorphic DNA analysis.
（扩增片段长度多态性）是一种新型的分子标记技术，目前已广泛应用于分子生物学及动植物育种等领域的研究。
AFLP marker is a new kind of technique, which is widely applied in the molecular biology, high mammal and plants in recent years.
本文阐述了PCR(基因扩增)技术的原理、基本操作方法，及其在兽医生物制品疫苗污染病毒检测中的应用。
This article expounds the principle of PCR technique, main methods of operations and its application to testing for virus pollution causing by veterinarian biological product.
目的探索随机扩增多态性DNA（RAPD）技术对大陆光亮钉螺遗传多态性研究的可行性，以获得不同地区光壳钉螺基因组DNA水平上的差异信息。
Objective Using RAPD technique to study genetic diversity of smooth-shelled Oncomelania hu- pensis in China and to gain more information on genome DNA level.
目的探索随机扩增多态性DNA（RAPD）技术对大陆光亮钉螺遗传多态性研究的可行性，以获得不同地区光壳钉螺基因组DNA水平上的差异信息。
Objective Using RAPD technique to study genetic diversity of smooth-shelled Oncomelania hu- pensis in China and to gain more information on genome DNA level.
应用推荐