细菌感受态的制备和转化；
感受态形成过程中，细胞可能发生了一系列的生理变化。
The physiologic change could take place in the process of forming competent cells.
枯草芽孢杆菌感受态细胞的质粒转化与质粒的分子构型有关。
The transformability of plasmid DNA in B. subtilis competent cell depends on its molecular structure.
目的：分析大肠杆菌JM109感受态形成因素，提高转化效率。
Objective:Analyzing the affecting factors of forming competent cells of Escherichia coli JM109 to increase transformation efficiency.
目的：建立一种高效、快速的大肠杆菌感受态细胞制备及质粒转化的方法。
Objective: To establish an efficient and rapid method for the preparation and transformation of competent cells from Escherichia coli.
连接产物转化至BL21感受态细胞，涂板、挑选单克隆进行菌落PCR。
The products were transformed into BL21 competent cells, coated plates, select monoclonal colony PCR.
研究了微束激光转化法用于棉花感受态萌动胚转化预培养的时间、高渗液对材料的处理等。
The recent advances about laser microbeam in plant transformation of exogenous gene, key techniques and applications are introduced.
研究了微束激光转化法用于棉花感受态萌动胚转化预培养的时间、高渗液对材料的处理等。
The research on manipulating manifold organisms cell alive using a laser micro beam with mechanics effect is reported.
利用LKB- 2277生物活性检测系统，测定了在生理盐水中，采用氯化钙法建立大肠杆菌感受态过程中的代谢热输出。
An LKB - 2277 bioactivity monitor was used to determine the metabolic heat output of the competence development of Escherichia coli HB101, which was induced with CaCl2 in physiological saline.
目的研究氯化铷法制备感受态细胞的转化效率和不同条件对其的影响。方法采用氯化铷法制备感受态细胞，用不同的质粒进行转化。
Objective to investigate the transformation efficiency and affecting factors in the preparation of competent E. coli cells by using rubidium chloride (RbCl) method.
目的研究氯化铷法制备感受态细胞的转化效率和不同条件对其的影响。方法采用氯化铷法制备感受态细胞，用不同的质粒进行转化。
Objective to investigate the transformation efficiency and affecting factors in the preparation of competent E. coli cells by using rubidium chloride (RbCl) method.
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