探讨不同培养基上三七花粉愈伤组织培养过程中总皂甙含量的变化情况。
The study aimed to discuss the content changing of total saponin during the callus culture of Panax notoginseng pollens on different media.
通过愈伤组织培养、悬浮细胞培养和原生质体培养方法对草坪草的一些种已建立较为完善的植株再生体系。
The plant regeneration systems of some turfgrass species have been established perfectly through callus culture, cell suspension culture, and protoplast suspension culture.
结论实验确定了冬凌草愈伤组织培养的最佳碳源种类、浓度及氮源比例，表明不同碳源、氮源对冬凌草愈伤组织生长和次生代谢物合成有显著影响。
The best carbon source and its concentration, nitrogen sources for the growth of Rabdosia rubescens (Henmsl. ) Hara callus and the synthesis of secondary metabolite were confirmed.
通过愈伤组织培养、悬浮细胞培养、原生质体培养等手段，对早熟禾属、黑麦草属、结缕草属和剪股颖属的一些物种已建立了较为完善的植株再生体系。
Plant regeneration system of the species of genus Lolium, Poa, Zoysia, Agrostis and Festuca were established by means of solid medium culture, suspension culture, and protoplast suspension culture.
本试验首次报道2，4，5-T和NAA对小麦愈伤组织培养反应的影响，特别是详细比较了它们与小麦组织培养试验中广泛应用的生长调节物质2，4-D之间的差异。
The main results showed as following:1. This is the first report about the effects of 2, 4, 5-T and NAA on wheat tissue culture, particularly in comparison with 2, 4-D in detail.
以紫果西番莲的花蕾为材料，进行组织培养愈伤组织诱导的研究。
Floral buds of Passiflora edulis Sims were used as explants in tissue culture.
用组织培养的方法使甜瓜子叶脱分化形成愈伤组织，继而在分化培养基上形成不定芽。
The method of tissue culture is used to induce the cotyledons of cucumis melon to become callus and then adventitious buds.
结果表明当年生枝条茎段组织培养春天比秋天成活率高，绿色致密的愈伤组织能够成苗。
The results showed that shoot segment in vitro survive rate is larger in spring than in autumn, and green dense callus could form plantlet.
通过优化提取工艺和筛选最佳植物生长调节剂浓度与配比，运用组织培养技术提高了罗布麻愈伤组织中黄酮含量。
Flavonoids content in callus of A. venetum were increased with tissue culture technology through optimizing extraction process and screening concentration and combination of plant growth regulators.
据此我们认为在甘蔗组织培养中2.4- D可能通过调节内源激素的水平及其相互作用，引起培养物中某些生理生化过程发生改变，从而进行脱分化和愈伤组织形成。
It appeared that the sugarcane leaf segments dedifferentiation and callus formation induced by 2.4-d May be related with the changes of endogenous levels of zeatin, zeatin riboside and ABA.
脱分化途径产生的生姜愈伤组织分化频率低，不利于 生姜组织培养。
The differentiation rate of callus produced by the pathway of de-differentiation was low, which was disadvantage to tissue culture of Zingiber offcinai Roscoe.
菘蓝再生体系培养表明：获得无菌苗的外植体是植物组织培养中最基础的一环，因此外植体的灭菌效果和愈伤组织与芽的诱导率直接影响着试验的进程。
The antisepsis result showed that: It is very important to get the asepsis explant. Because the sterilized effect can affect the growth of caudex and callus.
菘蓝再生体系培养表明：获得无菌苗的外植体是植物组织培养中最基础的一环，因此外植体的灭菌效果和愈伤组织与芽的诱导率直接影响着试验的进程。
The antisepsis result showed that: It is very important to get the asepsis explant. Because the sterilized effect can affect the growth of caudex and callus.
应用推荐