因此，与费劲的密度梯度离心方法相比，基于洗涤剂的细胞法分馏能简单快速地分离细胞组分（4）。
Thus, detergent-based cellular fractionation separates cellular components with greater ease and speed compared to a more laborious density centrifugation method (4).
用密度梯度离心法分离并计数支气管肺泡灌洗液（BALF）中的嗜酸性粒细胞；
The eosinophils in broncho-alveolar lavage fluid (BALF) were separated by density gradient centrifugation.
用改进的不连续蔗糖密度梯度离心法分离到五条带。
Five zones were separated by improved discontinuous sucrose gradient centrifugation.
对蔬菜绿叶通过组织匀浆，差速离心分离，密度梯度离心分离、纯化、提取叶绿体。
The green leaves of vegetables through tissue homogenate, differential centrifugation separation, density gradient centrifugation purify sample of chloroplast.
在较低的固相含量和较高的离心加速度下，浆料的分离现象明显，生坯中出现密度梯度。
The mass segregation phenomenon became more obvious at lower solid content and higher acceleration and there were density gradient in green compacts.
经等密度梯度离心分离，从褐煤、长焰煤、气煤和贫煤四种不同变质程度煤中获得了高纯度的有机显微组分。
Coal maceral concentrates of high purity were prepared from four different rank coals by Density Gradient Centrifugation method.
方法：取健康新西兰幼兔骨髓，密度梯度离心法分离骨髓间充质干细胞并进行体外扩增培养、成骨诱导分化及鉴定。
METHODS: Bone marrow was obtained from healthy New Zealand rabbits, by using density gradient centrifugation BMSCs were isolated and were then cultured in vitro.
方法不连续密度梯度离心法分离纯化大鼠肝贮脂细胞。
METHODS Rat lipocyte was isolated and purified with discontinuous density-gradient centrifugation.
采用蔗糖密度梯度离心法分离肝脏内质网。
Hepatic endoplasmic reticulum was isolated by differential centrifugation with sucrose density gradient.
方法：密度梯度离心法分离44例CHF患者和20例正常人PBMC，酶消化和差速贴壁法分离乳鼠心肌细胞。
Meathod: The experiment adopted density gradient centrifuging to separate PBMC of 44 patients with CHF and 20 normal person and enzyme digestion to separate neonatal rat cardiac myocyte.
方法：抽取兔髂骨及胫骨的骨髓液，经密度梯度离心法及塑料板贴壁培养法分离纯化出msc，并增殖。
Methods: MSC were harvested from iliac and tibias crest and obtained by using gradient centrifuge and adhesive culture methods.
用改良胶原酶消化技术，聚蔗糖密度梯度离心及手法挑拾方法从大鼠胰腺成功地分离出完整的胰岛。
This paper reports the successful isolation of islets of Langerhans from the rat pancreas using a modified collagenase digestion, Ficoll density gradient separation and hand-picking technique.
大鼠心肌细胞核采用差速离心和密度梯度离心分离提纯。
Velocity and isopyknic gradient centrifugation was employed to fractionate rat myocardial nuclei.
方法：抽取兔髂骨及胫骨的骨髓液，采用密度梯度离心法结合贴壁培养法分离纯化出MSC，并增殖。
Methods: MSC were aspirated from rabbit iliac and tibias crest and purified by gradient centrifuge and adhesive culture methods.
用密度梯度离心法分离后MEM培养基体外培养的人胚胎msc纯度高，生物活性好。
The purity of Human embryonic MSC which isolated by density gradient centrifugation and cultured by MEM culture medium in vitro is high. The activity of MSC is good.
密度梯度离心法分离肝内淋巴细胞；
The intrahepatic lymphocytes from each group were isolated by using density gradient centrifugation.
目前国外采用较多的有荧光激活细胞分选法、磁活化细胞分选法、免疫磁珠分离法、密度梯度离心法。
Various methods of enrichment exit:fluorescent activated cell sorting, magnetic-activated cell sorting, immunomagnetic beads, density gradient.
结论：利用密度梯度离心和贴壁筛选的方法可以分离hmsc，联合应用免疫细胞化学、细胞化学和电镜等技术手段鉴定HMSC的方法有效。
Conclusion The HMSC can be isolated with density-grad centrifugal and anchoring screening. The method to identify HMSC with immunocytochemistry, cytochemistry and electron microscopy is effective.
目的比较免疫磁珠法和密度梯度离心法分离纯化人外周血中性粒细胞的可靠性。
Objective To compare the reliability of immunomagnetic beads and density gradient centrifugation in purification and isolation of neutrophilic granulocytes from human peripheral blood.
方法使用密度梯度离心法分离不同年龄段脂肪间充质干细胞进行培养，保留贴壁细胞传代，分析脂肪间充质干细胞的纯度，观察细胞生长情况，检测其增殖活性、细胞周期。
The adherent cells were preserved to passage, the purity of ADSCs was analyzed by immunocytochemistry method, and cell growth was observed, then proliferation capability and cell cycle were detected.
方法使用密度梯度离心法分离不同年龄段脂肪间充质干细胞进行培养，保留贴壁细胞传代，分析脂肪间充质干细胞的纯度，观察细胞生长情况，检测其增殖活性、细胞周期。
The adherent cells were preserved to passage, the purity of ADSCs was analyzed by immunocytochemistry method, and cell growth was observed, then proliferation capability and cell cycle were detected.
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