方法：应用免疫组化法检测乳腺癌及相应癌旁组织tlr4、增殖细胞核抗原(pcna)的表达，TUNEL法检测组织中细胞的凋亡。
Methods: the expression of TLR4 and PCNA in cancer tissues and adjacent tissues were assessed by immunohistochemical method and the apoptosis was detected by the TUNEL method.
正如MTT法和计数pcna阳性细胞比例所显示：瘦素的给予与鼠np细胞的增殖成剂量依赖性关系。
Leptin administration led to increased proliferation of rat NP cells in a dose-dependent manner, as shown by MTT assay and by counting the percentage of PCNA-positive cells.
MTT法显示转染组的细胞增殖力低于未转染组和转染空载体组（P<0.05）。
The MTT showed cell proliferation ability was lower in the transfection group than in the non-transfection and mock-vehicle groups (P<0.05).
用MTT法分析顺铂对转染前后的8348细胞抑制增殖和诱导凋亡的能力。
Proliferation suppression and apoptosis of 8348 cells pre and post transfection induced by cisplatin were analysed by MTT methods.
通过扫描电镜、MTT测试法、考马斯亮蓝和碱性磷酸酶活性检测法，观察去抗原异种松质骨材料对细胞生长、增殖及功能表达的影响。
SEM, MTT assay and alkaline phosphatase (ALP) activity were carried out to observe the effect of AEXCB on cell growth, proliferation and functional expression.
方法：用液体或半固体培养法及流式细胞术测定血小板因子4对CD34阳性脐血细胞的增殖与分化的作用。
Methods: Liquid and semi solid culture systems as well as flow cytometry were used to study the effect of PF4 on the proliferation and differentiation of CD34+ cord blood cells.
用MTT法检测喜树碱对细胞增殖的抑制及有效浓度范围。
Inhibition on cells proliferation and effective concentrations of CPT are measured by MTT assay.
方法：应用MTT法观察体外壳寡糖和GAH对小鼠脾淋巴细胞细胞增殖的影响，应用巨噬细胞吞噬中性红试验观察壳寡糖和GAH对小鼠腹腔巨噬细胞吞噬功能的影响。
Methods:To observe the effects of the Chitooligosaccharidean and GAH on the lymphocyte proliferation of mouse by MTT assay and the phagocytosis of macrophages by Tuluylene red pinocyfic test.
结论肺癌相关抗原n 35是一种重要的肿瘤相关抗原，其功能很可能与肿瘤细胞无限制增殖活动有关。麦芽凝集素亲和层析法是纯化肺癌相关蛋白n 35简便有效的途径。
ConclusionThe lung cancer associated antigen N35 might be an important tumor associated antigen and related with the proliferation of cancer cells as a role of tumor cell growth regulator.
细胞生长曲线法和平板克隆法比较SP细胞和NSP细胞的增殖能力和克隆形成能力；
The colony-formation ability and proliferation ability between SP cells and NSP cells were compared in terms of plate colony assay and growth curve.
通过MTT法对单体化合物在犬血管平滑肌细胞模型上进行增殖抑制活性测试。
The purified compounds' inhibition against proliferation of dog vascular smooth muscle cells was also screened by MTT assay.
采用划线法和MTT法观察血管生成抑制素和反应停对淋巴管内皮细胞增殖和游走的影响。
Scraping line method and MTT were used to observe the effect of angiostatin and thalidomide on proliferation and migration of LEC.
采用MTT比色分析法检测细胞增殖情况。
MTT colorimetry was used to determine the proliferation of SW1116 cell line.
以显微分光光度法和流式细胞计观察经维甲酸（RA）或二甲亚砜（DMSO）处理HL-60细胞的DNA含量和细胞增殖的动态变化。
The alterations of DNA level and cell proliferation dynamics in HL-60 cells induced by RA or DMSO were observed.
通过MTT法观察淋巴细胞增殖，计算刺激指数(SI)。
Lymphocyte proliferation was observed with MTT for stimulation index (SI).
MTT法研究HCMV对体外培养涎腺导管上皮细胞增殖活性的影响；
The effects of human cytomegalovirus (HCMV) on the proliferation of HSG were investigated by MTT method in vitro.
分别采用台盘蓝拒染法及流式细胞术，在不同条件下测定细胞增殖的抑制率及细胞周期的分布。
Methods The cell growth was tested by trypan blue stain assay and the cell cycle was analysed by flow cytometry in the various conditions.
方法采用系统溶剂法从树舌灵芝得到一系列提取物，运用MTT法研究了各提取物对体外培养的人乳癌MCF-7细胞增殖的影响。
Methods All the extracts were obtained by the technology of solvent-refining, and MTT method was used to test the cytotoxicity of the extracts on breast cancer of MCF-7 cells.
采用MTT法检测绞股蓝多糖对体外培养的肿瘤细胞增殖的抑制作用。
The inhibitory effect of polysaccharide on tumor cells proliferation was assayed by MTT method.
方法：应用MTT（噻唑蓝）法检测汉黄芩素对于A549细胞增殖活性；
Methods:MTT chromatometry was employed to determine the influence of wogonin on proliferation of A549 cell.
MTT法检测细胞增殖抑制作用。
Inhibition of cell proliferation was determined by MTT assay.
方法采用人微血管内皮细胞（HMVEC），用MTT和记数法观察不同浓度的环磷酰胺对HMVEC的增殖和出芽抑制活性。
Methods Human microvascular endothelial cells(HMVEC) were cultured. MTT and number-calculating methods were used to observe cyclophosphamide on HMVEC′s proliferation and sprouts.
无论是曲面拟合法还是路径线法，各量计线上增殖误差可用时间多项式函数来表示，且多项式最高幂次数与量计线数目有关。
Propagation error for each gage can be expressed as polynomial of time and the highest power of polynomial ism-1, which mis the number of gages.
方法采用台盼蓝拒染法，在不同条件下测定细胞增殖的抑制率。
Methods The inhibitory rates of cell growth were tested by trypan blue stain assay in the different conditions.
结论改良MTT法在研究悬浮细胞增殖及药物毒性方面明显优于传统方法。
Conclusions Improved MTT assay had more advantages than the traditional one in studying the proliferation and cytotoxicity on suspended cell lines.
方法：抽取兔髂骨及胫骨的骨髓液，采用密度梯度离心法结合贴壁培养法分离纯化出MSC，并增殖。
Methods: MSC were aspirated from rabbit iliac and tibias crest and purified by gradient centrifuge and adhesive culture methods.
方法通过MTT法、台盼蓝拒染法绘制生长曲线观察姜黄素对CNE-2Z-H5细胞体外增殖的影响；
Methods The effects of the curcumin on proliferation in CNE-2Z-H5 cells in vitro were studied by MTT assay and trypan blue dye exclusion.
采用台盼蓝拒染法检测ATRA对SY5Y细胞的体外抗增殖作用；
Trypan blue exclusion was used to detect the antiproliferative effect of ATRA on SY5Y cells.
方法：抽取兔髂骨及胫骨的骨髓液，经密度梯度离心法及塑料板贴壁培养法分离纯化出msc，并增殖。
Methods: MSC were harvested from iliac and tibias crest and obtained by using gradient centrifuge and adhesive culture methods.
MTT法检测克隆球细胞和贴壁细胞的增殖能力和对化疗药的敏感性，并植入裸鼠皮下比较移植瘤形成能力；
MTT method was used to measure proliferation capacity and sensitivity to chemotherapeutic drugs, and tumorigenicity was measured by transplanted into the nude mice.
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