方法构建含tK基因的逆转录病毒表达载体，电穿孔法转化肺癌细胞A549。
Methods a retroviral vector containing TK gene was constructed and transduced into pulmonary adenocarcinoma cell A549 by electroporation.
在植物基因工程中，农杆菌质粒介导的遗传转化是比较完善与有效的基因转移方法，但在单子叶植物上应用较少。
The gene transformation by agrobacterium'plasmid is a perfect and effective method of transferring gene in the gene engineering, however, there is a little applied on the monocotyledons plants.
利用该转化方法，现已成功地获得了多种动植物细胞外源基因的转化。
Exogenous genes have been successfully introduced into a number of animal and plant cells by this transformation system.
脂肪酸去饱和酶基因，含该基因的载体，用该基因转化的植物，以及产生该植物的方法。
Fatty acid desaturation enzyme gene, vector contg said gene, plant transformed therewith and method for producing said plant.
本文从受体材料、转化方法、目标基因、影响因素、转基因遗传等多个方面综述了迄今为止高羊茅遗传转化研究所取得的进展。
In this paper, progresses in genetic transformation of tall fescue were reviewed in terms of recipient materials, transformation methods, target genes, influencing factors and transgene inheritance.
目前我国在甘蔗遗传转化研究中应用的研究方法主要是农杆菌介导及基因检转化。
The genetic transformation methods for sugarcane in China are mainly Agrogacterium-mediated transformation and gene gun transformation.
该文就植物抗病基因工程的原理、目的基因、转化方法等进行综述，并对植物抗病基因工程的应用前景做了展望。
The principle, targeting genes, transformation methods and view of application prospect of plant disease resistance genetic engineering were summarized.
与棉花体细胞胚胎再生途径相比，棉花茎尖分生组织培养植株成苗的时间短，培养方法简单，也不受基因型的限制，适合作为基因转化的受体系统。
In comparison with embryogenesis, it is easy to get plantlets from shoot apical meristems, and it is suitable and convenience to be used as transformation system.
笔者综述了近年来外源基因导入小麦的研究概况，阐述了外源基因导入的技术以及检测方法，分析了外源基因转化在小麦育种中的发展前景。
This report summarized the researching status of wheat exogenous gene transformation in recent years. The prospects of its applications in wheat breeding field was also analysed.
本发明还提供以采用基因导入法为特征的转化植物的制造方法。
The present invention also provides a process for preparing a transformed plant characterized in that the gene introduction method of the present invention is used.
本发明描述了导致获得12-15％转基因植物的高度改良的、可重 复性和一致的转化及再生方法。
The invention describes a highly improved, reproducible and a consistent method of transformation and regeneration that results in obtaining 12-15% transgenic plants.
现代育种手段进行果酒酵母的选育方法有诱变育种、杂交育种、原生质体融合、基因克隆和转化等技术。
The breeding methods of fruit wine yeast nowadays cover mutation breeding, cross breeding, protoplast fusion, and genetic cloning and transformation etc.
在植物基因工程中，农杆菌质粒介导的遗传转化是比较完善与有效的基因转移方法，但在单子叶植物上应用较少。
The gene transformation by agrobacterium'plasmid is a perfect and effective method of transferring gene in the gene engineering, however, there is a little apply on the monocotyledons plants.
目前用于油菜基因转化的方法有根癌农杆菌介导法、基因枪法、PEG法、激光微束穿刺法、显微注射法和花粉介导法等。
At present, there are several methods of transformation in rape, including tumefaciens-medidated genetic etc, particle gun, PEG untake, vialaser microbeam puncture and microinjection.
此外，还提供了一种含有此基因的表达载体、一种导入了该表达载体的转化体以及一种利用该转化体制备TRP - 1蛋白的方法。
Furthermore, an expression vector including the gene, a transformant into which the expression vector is introduced, and a process for producing the TRP-1 protein using the transformant are provided.
将反义蜡质基因和潮霉素抗性基因通过共转化方法导入了籼稻9311，并进行了初步检测。
The antisense Waxy gene and hygromycin resistant gene were co-transformed into Indica rice 9311 and passed the basic detection.
T - DNA标签法转化植物建立突变体库是植物功能基因组研究的重要而有效的方法。
Using T-DNA tagging approach for transforming plant is a powerful tool for the study of plant functional genome.
T - DNA标签法转化植物建立突变体库是植物功能基因组研究的重要而有效的方法。
Using T-DNA tagging approach for transforming plant is a powerful tool for the study of plant functional genome.
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