基因芯片检测结果与部分原位杂交结果进行比较并分析。
The results were compared with that of in-situ hybridization (ISH).
基因芯片检测慢性乙型肝炎不同证候的HBV前C区基因变异。
HBV gene mutation along different types of TCM syndrome are detected by microarray assay.
与现有的基因芯片检测技术相比,这种方法不仅结果可靠灵敏,而且简便快速、重复性好。
Compared with current gene chip detection technologies this method has the advantages of high reliability, sensitivity, easy manipulation and good reproducibility.
目的建立猪链球菌基因芯片检测方法,实现对猪链球菌种、致病血清型及毒力相关基因的同步检测和鉴定。
In order to develop a DNA microarray to detect Steptococcus suis species, its serotype and virulence-associated genes simultaneously, a set of probes specific for the target genes were designed.
我们主要介绍了基因探针检测法、PCR检测法和基因芯片检测法的原理、开发及其在食品微生物检测中的应用。
In this article we introduced the principle, development and application in food microbiological detection of three common molecular biology methods, gene probe, PCR and gene chip.
本文综述了甲型肝炎病毒的病原学、食品中甲肝病毒检测情况、ELISA方法和基因芯片检测甲肝病毒研究进展。
The research progression of etiology of hepatitis a, detected condition of hepatitis a from food, and diagnosis of hepatitis a by ELISA and gene chip were summarized.
本文综述了基因芯片技术在检测致病菌方面的应用、存在的问题及发展前景。
In this review the application, unsolved problems and prospect of the technology in pathogenic bacteria detection are introduced.
本发明涉及一种可制作标准曲线的临床定量检测基因芯片方法,属于生物技术领域。
The invention relates to a method of the clinical gene chip quantitative measurement for manufacturing a standard curve, and the method belongs to the biological technology field.
方法对91例乙型肝炎患者采用基因芯片技术,检测HBV6位点的自然变异。
Methods 6 sites mutations of HBV in 91 patients were detected by gene chip technique.
基因芯片技术以其具有高通量、快速灵敏、样品用量少等显著特点在病原菌检测中发挥着越来越重要的作用。
DNA microarray which is a high-throughput, rapid method and needs few samples has been considered as a potential approach to the detection of pathogenic bacteria.
基因芯片的杂交、洗涤、检测与分析。
Then the gene chip was hybridized, washed, detected and analyzed.
同时,本发明的方法可将基因芯片的高通量检测与定量检测结合,扩大基因芯片的应用范围。
Simultaneously, the method of the invention can combine the high flux measurement and the quantitative measurement of a gene chip, and the application range of the gene chip is enlarged.
目的研究基因芯片在检测结核分枝杆菌利福平和异烟肼耐药性中的应用。
Objective To study the application of gene chip in detecting Mycobacterium tuberculosis resistant to rifampin (RFP) and isoniazid (INH).
本发明作为基因芯片诊断的探针及PCR扩增检测序列,用于白色念珠菌的诊断。
The present invention is used as probe and PCR amplification detection sequence for gene chip diagnosis for candida albicans.
基因芯片是近年来兴起的基因检测新技术。
DNA Microarray is a new developed technology for gene detection in recent years.
方法:应用基因芯片、反转录PCR(RT-PCR)、流式细胞术等方法从分子和蛋白水平检测两种细胞系中CXCR4的表达情况。
Methods: Gene chip and RT-PCR were used to study expression of CXCR4 mRNA in two cell lines and the CXCR4 surface expression was also examined by flow cytometry analysis.
目前,商业化的检测设备价格昂贵,限制了基因芯片技术的推广和普及。
Nowadays, the high price of the commercial detector has prevented the gene chip technology from spreading and popularizing.
目的:制备人类致病病毒属水平高通量筛查用基因芯片,并通过检测黄病毒属病毒初步验证其筛查效果。
Objective: to establish a high throughput DNA microarray method for rapidly screening human viral pathogens, and to verify its effectiveness by detecting Flavivirus.
基因芯片技术已经成为检测不同状态下生物体基因变化的重要手段。
Gene chip has been an important way to detect gene changes of organism in different condition.
介绍了基因芯片的类型和制作原理。探讨了基因芯片在植物病害检测和转基因检测中存在的问题和应用前景。
Types and technological principles of gene chips were reviewed, and the problems and prospect of gene chips in plant disease testing and genetically modified organisms detecting were also discussed.
目的利用基因芯片技术进行常见革兰阳性细菌的细菌鉴定和耐药性检测。
Objective To study the clinical application of DNA chip for common Gram-positive bacteria identification and drug resistance detection.
结果与临床常规方法相比,耐药检测基因芯片鉴定指标的灵敏度均>96%,特异度均>98%;
Results The sensitivity of all identification indexes of DNA chip were higher than 96%, and all of their specificity were higher than 98%.
基因芯片技术是近年来出现的分子生物学与微电子技术相结合的最新DNA分析检测技术。
Gene chip technology is the latest DNA analysis and examining technology which combined molecular biology with microelectronics.
本文建立了一个基于连接酶检测反应的基因多态性并行检测系统,该系统应用连接酶检测反应与基因芯片技术。
Typing technology is the main method to evaluate gene polymorphism. The parallel typing system based on ligase detection reaction is (established) for gene polymorphism.
目的结合纳米放大芯片检测的专利技术,设计并制作适用于病原体快速检测的实用型基因芯片,以实现对临床常见感染病原体的快速、准确地检测和鉴定。
OBJECTIVE A practical gene chip which aimed to detect and identify pathogens rapidly and exactly is developed on the basis of patent technology of nano-enlargement-detection.
结论基因芯片技术检测HBV变异具有高通量、高灵敏等特点,对观察h BV基因突变有很高的临床应用价值。
Conclusions the gene chip technique is simple to perform and can be used as a rapid and high-throughput method. It has a high clinical value in detecting the HBV gene mutation.
结论基因芯片技术为黄热病病毒检测提供了一种早期、速、靠的方法。
Conclusion Microarray technology provides an efficient alternative for the detection of yellow fever virus.
方法:应用基因芯片技术对同一家系中的4例虚寒证患者与6名正常人进行基因表达谱检测。
Methods: Microarray technique was used for examination of the gene expression profiles in 4 cases of cold syndrome patient and 6 normal persons in a same family.
方法:应用基因芯片技术对同一家系中的4例虚寒证患者与6名正常人进行基因表达谱检测。
Methods: Microarray technique was used for examination of the gene expression profiles in 4 cases of cold syndrome patient and 6 normal persons in a same family.
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