本研究扩增得到的基因片段与报道的猪ghrh基因编码区序列完全一致。
Homogenious analysis showed that it was consistent with the reported porcine GHRH gene sequence.
目的:对不同粘附力变形链球菌临床分离株表面蛋白V区、P区编码基因进行序列测定。
Objective: To sequence V-region and P-region gene of surface protein of serotype c Streptococcus mutans clinical isolates with different adherent abilities.
经d NA序列分析表明所获基因含有全部前导序列,其成熟蛋白编码部分与从第一骨架区引物所克隆的序列相符。
DNA sequences analysis indicated that the cloned genes included the whole leader sequences and the mature Ig protein encoding regions.
分别设计MSX1、PAX9基因特异性引物,聚合酶链反应扩增全部外显子编码区和内含子-外显子剪接序列,产物纯化后直接测序。
Specific primers were designed for MSX1 and PAX9 respectively. Mutation analysis was performed by direct sequencing of all the coding exons and intron-exon boundaries.
在HCV的编码基因中,核心区(C区)序列具有高度遗传保守性,c基因表达产物具有良好的抗原稳定性。
Among HCV encoding gene, core region shows greater sequence conservation. Core gene expression has favorable antigen stability.
方法1。根据HSP70全长基因序列设计针对其编码区的引物,在下游引物的宁端加人MG7模拟表位的核昔酸序列。
Methods (1) The coding sequence of MG7 mimotope (GC23) was included in the reverse primer, and by PCR incorporated with HSP70 gene at the 3 terminus.
对分离到的HEV71阳性分离株进行VP1编码区基因扩增,核苷酸序列测定和同源进化分析。
And identified HEV71 isolates were performed by gene amplification of VP1 coding region, nucleotide sequencing and homology analysis of evolution.
同时,采用同源序列分离法,获得了小麦PSY基因的完整编码区序列。
Otherwise, the complete coding sequence of phytoene synthase gene (PSY) was isolated from the same wheat variety by using homologous sequence separation.
同时,采用同源序列分离法,获得了小麦PSY基因的完整编码区序列。
Otherwise, the complete coding sequence of phytoene synthase gene (PSY) was isolated from the same wheat variety by using homologous sequence separation.
应用推荐