尽管如此，目前互联网上已经在向那些有钱人推销检测基因变异的基因测试法。
Despite this, genetic tests for this gene variant are currently being marketed on the Internet for those who can afford them.
采用间接免疫荧光法(IFA)检测外源基因的表达。
The expression of exogenous genes were analyzed by indirect immunofluorescence assay (IFA).
采用间接免疫荧光法(IFA)检测外源基因的表达。
The exogenous gene expression was analyzed by indirect immunofluorescence assay (IFA).
采用单链聚合酶链反应(PCR)和测序法检测肿瘤组织中VHL基因的突变情况。
Mutation of VHL gene from tumor tissue was detected from tumor tissue by polymerase chain reaction (PCR) and direct sequencing.
目的：评价特异性甲基化pcr (msp)分析法检测抑癌基因高甲基化对结、直肠癌的诊疗价值。
Aim: To evaluate the clinical value of MSP (methylation specific PCR) in detection of tumor suppressor genes methylation in the colorectal cancer.
方法：应用聚合酶链反应（PCR）-双酶切法，对23例CMT1患者和30例正常人进行基因特异性连接片段的检测。
METHODS:Polymerase chain reaction(PCR) combined with restriction enzyme digest ion were used to detect gene specific junction fragments of the 23 CMT1 patients and 30 normal controls.
以大骨鸡为试材，采用RT-PCR法检测了MSTN基因在大骨鸡不同器官中的表达情况。
The present paper analyzed the expression of MSTN gene in different organs of Big Bone Chicken by RT-PCR.
应用多核细胞法检测外周血淋巴细胞HPRT基因突变率。
Methods HPRT gene mutation frequency was examined by the technique of multinuclear cell assay.
我们主要介绍了基因探针检测法、PCR检测法和基因芯片检测法的原理、开发及其在食品微生物检测中的应用。
In this article we introduced the principle, development and application in food microbiological detection of three common molecular biology methods, gene probe, PCR and gene chip.
方法采用DNA双向测序法直接检测类脂质蛋白沉积症一家系中患者及其父母、同胞、子女的ECM1基因。
Methods Bi-directional DNA sequencing was used to detect the ECM1 gene in patients with LP as well as among their parents, siblings and children.
应用免疫组化s - P法检测24对食管癌组织及相应正常食管组织中抑癌基因pten的蛋白质表达水平。
Expression levels of PTEN gene were detected by the SP immunohistochemical technique in the selected 24 pairs of tumor tissues and the related normal tissues.
另外，用免疫组化SP法检测癌组织和相应癌旁组织里PIK3CA基因的表达。
In addition, immnohis -tochemistry SP method was employed to test the expression of PIK3CA gene in carcinomas tissue and corresponding beside tissue.
方法应用rtP CR检测abc家族基因的转录表达，MTT法检测耐药性改变。
Methods Expression of ABC family genes was examined by RT PCR, and cell growth by MTT test.
并将外源基因alb亚克隆入该调控序列内，转染c 127细胞，经免疫组化法检测其表达情况。
Subclone the exogenous gene ALB into the regulating sequence for transfection of C127 cells.
目的了解采用双纸片协同法进行CTX - M酶的表型检测与多重pcr法检测基因型的相关性。
Objective to investigate the correlation between phenotypic confirmatory by double disc test and genotype of CTX-M-enzymes detected by multiplex polymerase chain reaction (PCR).
采用根癌农杆菌介导法将油菜CBF1基因转入拟南芥，筛选抗性转基因拟南芥植株，进行PCR检测和GUS染色。
CBF1 gene from rape was transformed into Arabidopsis thaliana by Agrobacterium tumefaciens-mediated method to screen its transgenic plants with resistance and conduct PCR detection and GUS staining.
直接测序法检测RT PCR产物的基因变异；
Mutation analysis was detected by direct sequencing RT-PCR products.
方法利用聚合酶链反应-序列特异引物(PCR -ssp)法，对189例银屑病患者和273例健康人的HLA - DQA1和DQB1等位基因进行检测。
Method Polymerase chain reaction sequence specific primers (PCR-SSP) method was used to analyze the frequencies of HLA-DQA1 and DQB1 alleles among 189 patients with PV and 273 healthy controls.
芯片法检测HCV基因型和测序法的符合率为75%。
In detection of HCV genotype, the coincident rate of microarray and sequencing was 75%.
用PCR法检测氨基糖苷类药物修饰酶基因。
The PCR method were performed to determine the genotypes of aminoglycoside modifying enzymes.
结果：经荧光显微镜、流式细胞仪及免疫印迹法检测，证实AIRE基因得到了稳定表达。
Results: Stable expression of AIRE was confirmed by fluorescence microscope, FACS and western blotting analysis.
实验结果表明，用改良的CTAB法提纯得到的大豆加工产品中的DNA完全可以满足PCR等分子生物学分析，而且定性PCR法能有效检测大豆及其加工产品中转基因成分。
The results showed that the DNA purified by the modified CTAB method was suitable for PCR and other molecular analysis, and the qualitative PCR was an effective way to screen the GMO.
目的：探讨利用新的高分辨率熔解曲线分析法检测结直肠癌中KRAS基因突变的可行性。
Objective: To evaluate the feasibility and sensitivity of detecting the mutations of KRAS in colorectal carcinoma patients with high resolution melting curve analysis.
近来，FDA已批准了应用基因试验来检测改变华法林代谢作用的等位基因，以指导进行该药物的适当给药。
The FDA has recently approved the use of gene testing for alleles that alter the metabolism of warfarin to guide in obtaining appropriate dosing for this agent.
PCR法检测氨基糖苷类修饰酶编码基因。
The PCR was performed to determine the genotypes of aminoglycoside-modifying enzymes.
从样品鳍条提取基因组dna，水平式淀粉凝胶电泳法检测同工酶。
The DNA samples were extracted from the caudal fin ray of samples.
以半定量RT-PCR法检测瘤细胞中HDAC1基因表达加以验证。
Semiquantitative RTPCR was used for verification of HDAC1 gene expression.
对异基因外周血造血干细胞移植的猕猴，用Y特异性序列分析法和性别染色体检测法于移植后7及14天均检测到雄性供者嵌合。
Male donor chimerism were found on day 7 and 14 after allogeneic stem cell transplantation by Y-specific sequence and chromosome karyotype analysis.
结论：一步法rt - P CR检测BCR - ABL融合基因有很好的灵敏度和特异性，操作简便、快速，特别适用于临床检测。
Conclusion: detection of BCR-ABL fusion gene by one step RT-PCR has excellent sensitivity and specificity. The method is simple and rapid, and is exactly used to clinic detection.
RFLP法检测维生素D受体（VDR）基因多态性。
Vitamin D receptor (VDR) gene polymorphism was detected using PCR-RFLP.
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