方法制备了包含87个心血管疾病相关基因探针的寡核苷酸芯片。
Methods Oligonucleotide microarrays consisting of 87 probes representing 87 human cardiovascular disease-related genes were constructed.
当前,报告基因显像的焦点是选择何种报告基因探针进行基因显像。
At present, the key problem is focused on the selection of what kind of reporter gene probe would be.
简述近年来重组受体、转基因动物、基因探针等基因工程技术在药物筛选中的应用。
The recent application of recombinant receptors, transgenic animals, genetic probe and other genetic engineering technology in drugs screening is introduced briefly.
绪论部分对现有的几种均相荧光基因探针技术进行了评述,指出了它们所存在的优势与不足。
The review is presented on several existing homogenous fluorogenic probes, and their advantages and disadvantages are discussed.
用基因探针技术检测食品中的有害微生物具有特异性强、灵敏度高和操作简便、省时等优点。
Detection of pathogenic and spoilage micro organism in foods by DNA probe has the advantages of high specificity, sensitivity, simple and time saving.
绪论部分对现有的几种均相荧光基因探针技术进行了评述,指出了它们所存在的优势与不足。
The thesis focuses on gene diagnosis of diseases, and six new homogenous fluorogenic probes are developed.
我们主要介绍了基因探针检测法、PCR检测法和基因芯片检测法的原理、开发及其在食品微生物检测中的应用。
In this article we introduced the principle, development and application in food microbiological detection of three common molecular biology methods, gene probe, PCR and gene chip.
用9个玉米和水稻线粒体基因探针与酶切条带杂交发现,不育系与保持系线粒体DNA的相同大小酶切片段含有相似的顺序。
Homologous sequences of mtDNA were found between MS and maintainer lines by using 9 probes of maize and rice to hybridize with digested mtDNA.
每一个探针与一种特定基因的RNA绑定——它含有了一整套化学物质和基质的集合。
Each probe binds to the RNA of a specific gene-the one that contains a complementary set of chemical units, or bases.
他们利用DNA探针阵列(即基因芯片)对客户的DNA突变进行预诊断。
They have employed arrays of DNA probes, known as gene chips, to look for pre-identified variations in their clients' DNA.
这一版本检测套装的探针中含有从5,700种病毒中采集的178,000个病毒基因序列,以及从数以千计的细菌中采集的785,000个细菌基因序列。
This version contains probes representing about 178, 000 viral sequences from some 5, 700 viruses, and about 785, 000 bacterial sequences from thousands of bacteria.
此外,鉴于肽核酸独特的理化和生物学性能,人们正尝试将其用于生物分子工具、反义和反基因制剂、分子探针和生物传感器。
In addition, the unique chemical, physical and biological properties of PNAs have been exploited to produce powerful biomolecular tools, antisense and antigene agents, molecular probes and biosensors.
结论:利用PCR扩增产物制备基因芯片探针是一种快速、简便、实用的方法。
Conclusions: Using PCR amplification products to prepare gene chip probe was a quickly, simple effective method.
本文综述了CYP2D6的结构特点,底物,探针药物,影响其活性的因素,基因多态性以及它与疾病的关系。
In the Present article, the characterization, substrates, probe drugs, factors affecting its activity, genetic polymorphism and the relation between CYP2D6 and several diseases were reviewed.
人工核酸酶是分子生物学及基因工程的重要工具,它可以作为研究核酸结构和功能方面的探针。
Artificial nuclease is an important tool in molecular biology and genetic engineering, it is also used as a kind of nucleic acid structure and function probe.
将探针固定在特制的玻片上制作成基因芯片;
The gene chip was prepared through the probes printed onto special glass slides.
结论PNA与靶序列dna结合有高度的特异性,选用PNA作探针,可提高压电基因传感器检测的特异性。
Conclusion PNA combining with the DNA of target sequence had a highly specificity. Choosing PNA as the probe may improve the determined specificity of piezoelectric gene sensor.
根据间日疟原虫环子孢子表面蛋白(CSP)基因序列研制了PCR/DNA探针检测系统。
Based on the gene sequence of circumsporozoite protein(CSP) of Plasmodium vivax , the PCR/DNA probe detection system was prepared.
方法根据APP的基因序列,设计并合成引物和荧光标记探针。
Methods According to the specific sequence of APP genes, the primers and the fluorogenic probe were designed and synthesized.
对天然核酶的改造和人工核酶的筛选使核酶的催化反应谱得到了扩展,也为核酶作为基因治疗药物和核酸探针提供了多种选择。
Its catalytic repertoire expanded by modification and in-vitro-selection of nucleic acid libraries endows ribozymes a great potential as gene therapeutic agents and nucleic acid probes.
我们自己制备的LZF—I探针,能与鱼类基因组DNA中的简单重复序列杂交形成杂交分子。
The oligonucleotide probe LZF-I, which is synthesized by us, can form thehybrid molecules with the simple repetitive DNA of three species of fishes.
在国内外首次利用抗体探针获得了植物抗病蛋白编码基因的部分序列,为进一步克隆全长的API蛋白基因奠定了基础。
It is the first report of obtaining partial sequences of plant disease resistance gene by using antibody probe in the world, which provides a basis of cloning the whole gene encoding API protein.
纳米粒子标记DNA探针的研究是纳米技术、基因组学、DNA检测等学科交叉的研究热点。
Nanoparticle tagging DNA probe, which combines of nano-technology, gene project and DNA detection, is the focus of recent research field.
本发明作为基因芯片诊断的探针及PCR扩增检测序列,用于白色念珠菌的诊断。
The present invention is used as probe and PCR amplification detection sequence for gene chip diagnosis for candida albicans.
试验结果表明,设计的寡核苷酸探针具有一定的特异性,能很好的检测乳酸杆菌中SLP基因。
The result showed that the probe above could effectively detect the SLP gene with a certain specificity.
标准的探针集注释是以基因为中心,也就是将探针集与特定的基因关联起来,而且探针集的表达谱最终会被翻译成基因表达谱。
Standard Affymetrix probe set annotation is gene-centered, i. e. probe set is precisely linked to gene, and probe set expression is interpreted as gene expression.
设计探针,应用人多组织杂交膜分析该基因的组织表达谱。
The gene was analyzed by making use of bioinformatics and its expression spectrum was studied by using multiple tissue Northern blot.
设计探针,应用人多组织杂交膜分析该基因的组织表达谱。
The gene was analyzed by making use of bioinformatics and its expression spectrum was studied by using multiple tissue Northern blot.
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