• 活体采屠宰场采卵的优质卵母细胞数和囊胚差异显著

    There was significant difference on recovered high quality oocytes and blastocyst rate between from ovum pick-up and from slaughterhouse.

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  • 小鼠孤雌囊胚氯化锶饲养最高76.21 %;

    Mice blastocyst rate of parthenogenetic embryos in SrCl2 plus feeder cells group was highest 76.21%;

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  • 结果表明:胎纤维细胞MEF培养囊胚和胚胎细胞数38

    The result showed that the mouse embryonic fibroblasts MEF group achieved significantly higher blastocyst formation rate 38.

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  • 结论表明生长期的细胞供体细胞融合囊胚显著高于经过接触抑制处理血清饥饿处理的细胞。

    Through the data we found that growing cells do donor cell fusion rate and blastocyst rate were significantly higher than after treatment and contact inhibition in cells treated with serum starvation.

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  • 添加新生牛血清KSOM与胚胎培养液G1/G2有效克服昆白小鼠2-细胞阻滞,能得到较高囊胚

    The KSOM medium with 10% NBS and medium G1/G2 were effective to overcome the 2-cell block, and the blastocysts rate of the two groups were high.

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  • 结果表明卵裂囊胚囊胚孵出而言,壁颗粒细胞单层颗粒细胞单层组之间无显著差异(P>0.05);

    The results indicated that there were no notable difference between granulosa cell monolayers group and cumulus cell monolayers group for the cleavage, blastocyst and hatched rate (P>0.05).

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  • 受精卵血清FBSOCS培养液中的囊胚发育明显高于在血清培养基中的囊胚发育

    Presumptive zygotes were cultured in mSOFaa medium supplemented with OCS or FBS had higher blastocyte yield than that in culture medium with free serum.

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  • 经统计分析,早期囊胚存活极显著高于桑椹胚(P<0.01)。

    The survival rats of early blastocyst groups were significantly higher than the morula groups (P<0.01).

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  • 目的探索体外培养辅助孵化技术体外发育小鼠囊胚影响

    Objective: To study the influence of in vitro culture and assisted hatching on the hatching efficiency of mice blastocysts cultured in vitro.

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  • 囊胚细胞体外培养,添加10%的血清细胞的贴壁最高

    When culturing blastodermal cells of chicken in vitro, the cell attachment rate is maximum with 10% fetal calf serum.

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  • 相同剂量剂量,处于4 ~8细胞胚胎存活细胞囊胚早期畸形低。

    Embryos at 4 ~ 8 cell stage showed higher survival rate and lower abnormal embryo rate than embryos at multicellular stage or early gastrula stage when radiated at the same dose and dose rate.

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  • 结果输卵管积液组的囊胚孵出低于无积液组,囊胚培养液中的MMP-9TIMP-1低于无积液组,并剂量依赖性,MMP-9/TIMP-1随积液浓度增加而降低

    Results: Hydrosalpinx fluid significantly decreased the hatching rate of blastocysts and the concentrations of MMP-9 and TIMP-1 both in blastocysts and culture medium, in a dose dependent manner.

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  • 说明了更换培养基不利于其体外囊胚发育

    The parthenogenesis efficiencies of bovine, ovine in vitro maturation(IVM) oocytes were also studied between ionomycin, electric activation and replaced medium.

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  • 说明了更换培养基不利于其体外囊胚发育

    The parthenogenesis efficiencies of bovine, ovine in vitro maturation(IVM) oocytes were also studied between ionomycin, electric activation and replaced medium.

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