结果获得库容量约为107噬菌体抗体库。
Results The ScFv phage repertoire with capacity of 107 was obtained.
目的建立一种测定噬菌体抗体相对亲和力的方法。
Objective To establish a method for the measurement of relative affinity of phage antibodies.
目的构建人源抗红细胞A抗原单链噬菌体抗体库。
Objective To construct a phage display library of human single-chain Fv antibodies against blood group a substance.
目的构建人噬菌体抗体组合文库，筛选人单克隆抗体。
Objective To construct human phage antibody library and produce human monoclonal antibodies.
结论噬菌体抗体库技术是研制人源性单抗的有效途径。
Conclusion Phage surface display technology is a useful method to produce human monoclonal antibody against CEA.
目的评价菌落pcr法鉴定噬菌体抗体库阳性重组率的可靠性。
Objective To evaluate the reliability of colony PCR in identifying the recombinant clones selected from phage antibody libraries.
本研究建立了一套膜生物反应器系统，并首次用于噬菌体抗体的生产。
Here a membrane bioreactor system was set up, and was used to produce phage antibody for the first time.
结论：硫氰酸盐洗脱法用于噬菌体抗体的筛选有助于成功获得期望的高亲和力抗体。
Conclusion:Thiocyanate elution is a powerful and effective method to help obtain the Phabs with high affinity from phage antibody library.
本研究报道一种基于固定化金属亲和层析(IMAC)的噬菌体抗体库液相筛选方法。
In this study, a new method based on immobilized metal affinity chromatography (IMAC) for screening phage antibody library against antigen in solution was reported.
以固相化的SARS抗原淘筛抗体库，并用ELISA检测噬菌体抗体结合SARS病毒的特异性。
Antibodies against SARS virus were screened by biopanning with immobilized virus antigen. The binding specificity of phage antibody to SARS virus was detected by ELISA.
结论：利用噬菌体抗体库技术筛选可得到与破伤风外毒素特异性结合的人抗破伤风类毒素基因工程抗体。
Conclusion: Human genetic engineering antibody, which is able to react specifically to tetanus toxin, is obtained through screening phage antibody library.
采用正常人和白血病患者的白细胞对人源噬菌体抗体库进行淘选，以获得对两种细胞表面蛋白特异的抗体。
A large human phage antibody library was subjected for panning with leukocytes from healthy donors and leukemia patients to select for specific antibodies against leukocytic surface proteins.
将4个鼠源噬菌体抗体克隆和1个人源噬菌体抗体克隆偶联到羧基终止的硅片表面，制成分析型模型芯片。
A model analytical chip was prepared by coupling 4 phage antibody clones from mouse and 1 clone from human to the surface of carboxyl terminated silicon.
目的克隆白癜风患者淋巴细胞中原始配对的自身抗体基因，构建噬菌体抗体库并筛选针对黑素细胞膜抗原的特异性抗体。
AIM: To obtain in situ pairing of the variable region genes of the heavy and light chains of vitiligo patients' lymphocytes, then construct and screen a human ScFv phage antibody library.
以该融合蛋白为固相抗原，对噬菌体抗体库进行3轮淘筛，并对所获阳性克隆进行抗原结合活性测定和DNA序列测定。
Specific antibody was screened by 3 rounds of panning of phage antibody library with the fusion protein. The antigen binding activity and DNA sequences of positive clones were determined and analyzed.
噬菌体抗体库技术是抗体基因文库技术和噬菌体表面展示技术相结合形成的一项新技术与方法，在生物科学领域极具潜力。
The phage antibody library technology is a novel method with many potential applications, consisting of antibody library and phage surface display technology.
结论利用噬菌体抗体库技术获得了人源性抗角蛋白单链抗体，为临床应用研究提供了具有更广阔应用前景的人源小分子抗体。
Conclusion Human anti-keratin scFv is obtained from a large phage antibody library, which will benefit to the application of anti-keratin antibody to clinical research.
为了构建人源性抗脑胶质瘤抗体库，我们必须先从患者的血液内检测到抗脑胶质瘤抗体，才能为建立人源性噬菌体抗体库奠定基础。
We must prove that there is really brain glioma antibody in the blood of patients, then we can establish the base for constructing humanized phage antibody library.
整个筛选过程中抗原与抗体的结合在液相中完成，不仅消除了固相介质对抗原表位的影响，也更有利于噬菌体抗体与抗原的充分作用。
The binding process of antigen and antibody completed in the solution, which doesn't have the shortage of conformational changes encountered in the immobilized screening.
目的：构建噬菌体人源抗独特型抗体库。
AIM: To construct phage human anti idiotypic antibody library.
噬菌体表面展示技术广泛应用于疾病诊断、特异性抗体及蛋白药物生产等。
The phage surface display technology is widely used in disease diagnosis, specific antibody, and protein medicine production.
结论错配pcr结合噬菌体展示技术是提高单链抗体亲和力的一种有效且简便的手段。
Conclusion Error-prone PCR is an effective and simple method for affinity maturation of antibodies isolated from a phage antibody library.
结论：异种血管内皮生长因子基因重组T7噬菌体疫苗可打破机体对自身vegf的免疫耐受，诱导产生较高水平的特异性抗vegf抗体。
Conclusion: Recombinant T7 phage vaccine expressing xenogenic VEGF can break immunologic tolerance against self-VEGF and induce the producing of specific anti-VEGF antibody.
利用组合库和噬菌体展示技术，我们证明半抗原特异性抗体确实存在有外围载体的某些遗迹。
By using combinatorial library and phage display technologies on a hapten-specific antibody we were able to demonstrate that a peripheral carrier imprint indeed exists.
用辅助噬菌体m 13k07感染转化菌，以挽救出噬菌体形式的抗体库。
The transformed cells were infected with M13K07 helper phage to produce a phage form of library.
方法利用RTPCR 方法扩增抗体可变区基因并表达于噬菌体表面。
Methods The genes encoding antibody variable regions were cloned by RT PCR from hybridoma cells and expressed on bacterial phage surface.
建立杂交瘤单抗亲和层析纯化抗原、抗原体外致敏淋巴细胞和RT-PCR克隆人抗体基因及噬菌体呈现技术构建人源抗体库的策略。
Strategy was established for construction of repertoire antibody library with affinity chromatography purifying antigen, antigen immunizing human lymphocytes, RT-PCR and phage display technology.
通过噬菌体肽库技术分析抗体所识别的抗原表位。
The mAb epitope was also analyzed using peptide phage display technology.
目的制备高活性的可溶性抗人血管生长素噬菌体基因工程抗体。
Objective To prepare the high effective and soluble genetic engineering antibody of anti-human angiogenin.
目的制备高活性的可溶性抗人血管生长素噬菌体基因工程抗体。
Objective To prepare the high effective and soluble genetic engineering antibody of anti-human angiogenin.
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