研究人胎肝干细胞体外分离纯化方法，并对其进行初步鉴定及生物学特性分析。
To explore the methods of isolation and purification of human fetal liver stem cells in vitro and analyze the bionomics.
目的探讨超声引导肝内结节细针活检及甲胎蛋白（AFP）轻度升高在肝癌早期诊断中的价值。
Objective To research the value of ultrason ographic-guided fine needle biopsy of liver nodular lesion and low level elevation of serum alpha-fetoprotein(AFP) in early diagnosis of liver cancer.
方法：采用胶原酶灌注法及剪碎消化法分离大鼠胎肝干细胞，并用含10%优等胎牛血清的H -DMEM培养液培养。
Methods Collagenase perfusion method and mechanical cutting method were used to isolate HSCs from rat fetal liver which were then cultivated by H-DMEM containing 10% fetal bovine serum.
方法：采用胶原酶灌注法及剪碎消化法分离大鼠胎肝干细胞，并用含10%优等胎牛血清的H -DMEM培养液培养。
Methods Collagenase perfusion method and mechanical cutting method were used to isolate HSCs from rat fetal liver which were then cultivated by H-DMEM containing 10% fetal bovine serum.
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