新基因进行原核表达并纯化蛋白。
目的克隆srg基因、原核表达并鉴定。
目的克隆人颗粒溶素基因并进行原核表达。
Objective To obtain recombinant human granulysin using prokaryotic expression system.
目的构建人胃动素受体基因的原核表达载体。
Objective to construct expression plasmid of human motilin receptor.
目的克隆人TRAIL基因，构建其原核表达载体。
To clone gene of the TRAIL and construct its prokaryotic expression vector.
原核表达的蛋白质无论在沉淀中还是在上清中均有表达。
The protein of procaryotic expression exists both in the supernatant fluid and the sediment.
目的：克隆人MAGE3基因并进行原核表达和分离纯化。
AIM: to clone human MAGE 3 gene, to induce its prokaryotic expression and to purify the protein.
方法在原核表达系统中高效表达重组人3型腺病毒六邻体蛋白。
Methods Under the optimum expression conditions, the hexon protein of human type 3 adenovirus was efficiently expressed in e.
结论原核表达系统可有效克隆较高纯度的CHM - I基因。
Conclusion The more effective CHM-I clone with high purity could be expressed by procaryotic expression system.
目的：考察原核表达的重组人NDRG2蛋白对肿瘤细胞的作用。
Objective: To study the effects of prokaryotically expressed recombinant human NDRG2 on tumor cell lines.
目的构建凋亡素原核表达系统，以制备抗原物质凋亡素融合蛋白。
Objective to construct an apoptin expression system to produce an antigen, apoptin fusion protein.
目的构建土拨鼠肝炎病毒核心蛋白质粒并进行原核表达、抗体制备。
Objective To construct the prokaryotic expression plasmid expressing woodchuck hepatitis virus core antigen (WHcAg) and prepare polyclonal antibodies.
目的改构高致病性禽流感病毒血凝素基因，建立有效的原核表达体系。
Objective To modify the HA1 gene of high pathogenic avian influenza virus (HPAIV) and to build an efficient prokaryotic expression system of it.
目的构建结核杆菌分泌蛋白mpt53原核表达载体并进行表达和纯化。
Objective to construct a expression vector of MPT53 of mycobacterium tuberculosis and identify and purify the protein in the e.
通过优化原核表达条件，确定了原核表达的最佳诱导时间和诱导剂浓度。
After optimizing prokaryotic expression conditions, we determined the optimum inducement time and concentration of IPTG.
目的原核表达重组人甘露聚糖结合凝集素（MBL）糖识别域（CRD）。
Objective To express the recombinant carbohydrate-recognition domain (CRD) of human mannan-binding lectin(MBL)in E. coli.
目的：构建食管癌相关基因2ECRG2原核表达质粒，在大肠杆菌e。
Objective: to construct a prokaryotic expression vector containing esophageal cancer related gene 2 ECRG2 and observe its expression in Escherichia coli e.
汪静。李官成。童永清鼻咽癌人源抗独特型基因工程抗体的原核表达及鉴定。
Wang J. Li GC Expression, purification and identification of the human anti-idiotypic single chain antibodies against nasopharyngeal carcinoma in E. coli.
目的建立HPV16 L 1蛋白原核表达系统的纯化方法，纯化目的蛋白。
Objective To establish a method of purification of HPV16L1 protein expressed in a prokaryotic system and obtain the purified protein.
完成了NKG2D的原核表达载体的构建，表达并纯化了重组NKG2D蛋白。
The NKG2D prokaryotic expression vector was successfully constructed. The recombinant NKG2D is expressed and purified.
结论原核表达的截断型人可溶性CD 4 0 L具有抑制肿瘤细胞生长作用。
Conclusion the soluble human CD40L truncate expressed in prokaryotic cells showed inhibiting effect on the growth of tumor cells.
目的构建柔嫩艾美耳球虫子孢子表面抗原原核表达载体，并且在大肠杆菌中表达。
Objective To construct the prokaryotic expression vector of the sporozoite surface antigen gene of Eimeria tenella GZ strain and expression in Escherichia coli.
本研究成功利用原核表达系统实现了对CT抗原NY-ESO-1的可溶性表达。
So human CT antigen NY-ESO-1 could be successfully expressed in prokaryotic expression system.
目的：构建人热休克蛋白70（HSP 70）的原核表达载体，诱导其表达并纯化。
Objective: To construct the prokaryotic expression vector of human heat shock protein 70(HSP 70) and to induce the expression and purification of HSP 70 in vitro.
目的构建针对APRIL的免疫抑制分子，并对其原核表达蛋白进行纯化及活性筛选。
Objective To construct, express, purify and screen immunosuppressive molecule against human soluble APRIL (a proliferation-inducing ligand).
试剂盒中96孔板中的包被抗原为原核表达的重组P 30蛋白，其具有良好的抗原性。
The coating antigen in a 96 pore plate in the kit is the prokaryotic expression recombinant P30 protein, and has good antigenicity.
原核表达并纯化单纯疱疹病毒特异性糖蛋白抗原，用于建立单纯疱疹病毒的临床检测方法。
To express and purify the specific glycoprotein antigens of herpes simplex virus (HSV) as used in HSV clinical detection.
目的构建人鸟氨酸脱羧酶抗酶(OAZ)突变基因，重组至原核表达载体中并表达出重组蛋白。
Objective To clone human ornithine decarboxylase antizyme (OAZ) mutation gene and express its recombinant protein.
目的构建人鸟氨酸脱羧酶抗酶(OAZ)突变基因，重组至原核表达载体中并表达出重组蛋白。
Objective To clone human ornithine decarboxylase antizyme (OAZ) mutation gene and express its recombinant protein.
应用推荐