本发明也提供结合至该TF的人源化抗体及其片段。
Also provided are humanized antibodies and fragments thereof that bind to the TF.
人源化抗体解决了抗体鼠源性的问题，但CD3单抗激活T细胞引起首剂效应的问题仍然存在。
The immunogenicity of 12F6 in humans has been reduced though humanization. But the first-dose reaction associated with T cell activation by anti-CD3 Abs still exists.
采用化学治疗药物(贝伐单抗，是中和血管内皮生长因子的人源化抗体)治疗可导致蛋白尿和肾功能损害。
Treatment with the chemotherapeutic agent bevacizumab, a humanized mab that neutralizes vascular endothelial growth factor, can lead to proteinuria and renal damage.
曲妥珠单抗是一种人源化抗体，用于靶向和阻断her2的功能，而后者是由特异基因编码的一种蛋白，具有致癌潜能。
Trastuzumab is a humanised antibody, designed to target and block the function of HER2, a protein produced by a specific gene with cancer-causing potential.
去糖基化后，小麦PGTP与天然纯化的 PGTP产生的抗体不发生相互作用。
After deglycosylated, wheat PGTP could not interact with antibody produced by purified native PGtP from wheat.
方法：选用特异性抗体，用免疫组化的方法检测了10例正常头皮标本中五种神经肽的表达情况。
Method: The expression of five neuropeptides (NPs) were detected in scalp specimen of 10 normal people by using immunohistochemical staining method with specific antibodies.
抗体的多样化，不仅形状的免疫记忆的剧目，而且自身抗体。
Antibody diversification not only shapes the repertoires of the immunological memory, but also those of auto-antibodies.
TACE抗体识别内源性的TACE蛋白，也有由差异糖基化导致的多余条带。
TACE Antibody detects endogenous levels of TACE protein. Additional bands result from differential glycosylation.
抗体生物素化后活性保持较好，生物素化效率较高。
The biological activity and the biotinylation efficiency of antibody keeps well after biotinylation.
以固相化的SARS抗原淘筛抗体库，并用ELISA检测噬菌体抗体结合SARS病毒的特异性。
Antibodies against SARS virus were screened by biopanning with immobilized virus antigen. The binding specificity of phage antibody to SARS virus was detected by ELISA.
去糖基化的小麦PGIP不能与纯化的天然PGIP产生的抗体发生免疫学反应，可能是由于小麦PGIP中寡糖链为抗原决定部位。
After deglycosylated, wheat PGTP could not interact with antibody produced by purified native PGtP from wheat. It is the glycans of wheat PGIP that probably fonn the epitope.
该方法体外模拟了抗体亲和力成熟的过程，使得到的抗体不但可以完全的人源化，而且可以具有较高的亲和力。
Because this method was to simulate the process of antibody maturation in vivo, the obtained antibodies was not only fully humanized but also with relatively high affinity.
二期的CheckMate- 140试验正在探索用nivolumab治疗经cd 20抗体和烷化剂治疗后进展的滤泡性淋巴瘤。
The phase II CheckMate-140 trial is exploring nivolumab (Opdivo) for patients with follicular lymphoma following progression on a CD20 antibody and an alkylating agent (NCT02038946).
在免疫印迹和免疫组化实验中，使用一个封闭试剂可以评估这个抗体反应的特异性。
Use as a blocking reagent to evaluate the specificity of antibody reactivity in Western blotting and immunohistochemistry protocols.
将其作为免疫组化实验中评估抗体特异性反应的封闭试剂。
Use as a blocking reagent to evaluate the specificity of antibody reactivity in immunohistochemistry protocols.
结论我国主要的商业化HIV抗体诊断试剂产品检测不同基因型抗体的能力无明显差异。
Conclusion The capacity of commercial HIV diagnostic kits to detect antibodies against different HIV genotypes may not be significantly different.
在免疫组化方法中，使用一个封闭试剂可以评估这个抗体的特异性反应。
Use as a blocking reagent to evaluate the specificity of antibody reactivity in immunohistochemistry protocols.
方法用免疫组化SABC法检测五种单克隆抗体，分别使用水煮法、微波法、高压法修复抗原，从中选出最佳修复方法。
Methods Check five kinds of antibody with immunohistochemistry SABC method and chose the best antigen retrieval method among poach method, microwave method and high pressure method respectively.
在免疫组化实验步骤中，使用一个阻断试剂去评价抗体活性的特异性。
Use as a blocking reagent to evaluate the specificity of antibody reactivity in immunohistochemistry protocols.
为了降低人抗鼠抗体反应，获得满意的免疫原性，将模拟人卵巢癌抗原的抗独特型单链抗体人源化。
In order to reduce the human anti mouse antibody response and obtain optimal antigenicity, anti idiotype single chain which mimicking ovarian cancer antigen has been humanized.
本发明提供一种CD86人源化单克隆抗体，其相关基因序列和表达系统。
The invention provides a CD86 humanized monoclonal antibody, an associated gene order and an expression system thereof.
掌握抗体最佳的修复方法能极大提高免疫组化结果的阳性率及准确率。
It can significantly improve the positive rate and accuracy of immunohistochemistry staining results to master the best antibody repairing method.
结果RSV地方株G蛋白在痘苗病毒中获得较好的表达，表达产物的糖基化程度较高，且该重组病毒免疫家兔可诱发特异性抗体产生。
Results The RSV G glycoprotein was expressed by the recombinant vaccinia virus, which was determined by Western blot with anti-G protein specific monoclonal antibody.
鼠单抗识别单泛素、多泛素和泛素化蛋白，这一抗体可能会与重组NEDD8发生交叉反应。种属特异性：所有种属。
Ubiquitin (P4D1) Mouse mAb detects ubiquitin, polyubiquitin and ubiquitinated proteins. This antibody may cross-react with recombinant NEDD8. Species Reactivity: All Species Expected.
超纯正常羊血清在发光和荧光免疫组化和免疫细胞化学方法中作为封闭液和抗体稀释液使用。
Premium quality normal goat serum for use as blocking reagent and antibody diluent for chromogenic and fluorescent immunohistochemical and immunocytochemical assays.
本研究报道一种基于固定化金属亲和层析(IMAC)的噬菌体抗体库液相筛选方法。
In this study, a new method based on immobilized metal affinity chromatography (IMAC) for screening phage antibody library against antigen in solution was reported.
使用抗KDR单克隆抗体（3G9），通过免疫荧光、免疫组化检测KDR在T24细胞及瘤体中的表达；
Anti-KDR McAb(3G9) was used to measure the expression of KDR (kinase insert domain-containing receptor) in T24 cells and tumor tissue by immunofluorescence and immunohistochemistry.
在细胞培养上清和小鼠腹水抗体的检测中，其灵敏度比经典的醛化致敏血球提高4～8倍。
The sensitivity of the test was proved to be 4—8 times as sensitive as that of classical passive hemagglutination test.
在细胞培养上清和小鼠腹水抗体的检测中，其灵敏度比经典的醛化致敏血球提高4～8倍。
The sensitivity of the test was proved to be 4—8 times as sensitive as that of classical passive hemagglutination test.
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