材料和方法：采用酶辅助的显微分离法分离新生牛眼ipe细胞，酶消化法分离角膜内皮细胞，进行体外细胞培养。
Material and Methods: From bovine eyes IPE cells were isolated with enzyme-assisted microdissection, corneal endothelium cells with enzyme digestion and then were cultured in vitro.
结果显微分离后弹力膜及内皮细胞层联合延迟消化培养法，体外培养牛角膜内皮细胞成功率高。
Results in the culture of BCECs in vitro, the methods of separating posterior elastic layer and endothelium of cornea under microscope, combined with delay digestion proved to succeed easily.
用HSV- 1的ACV耐药株感染兔角膜造模，分别用抗生素17997或ACV局部给药治疗，以局部病损及病毒分离判断疗效。
The efficacy of 17997 in treatment of ACV resistant HSV 1 induced rabbit keratitis was evaluated by lesion score and virus isolation.
方法置泪道探针于翼状胬肉颈部下，由翼状胬肉头部舆角膜之间缓缓擀出，使翼状胬肉顾部舆角膜完全分离。
Methods A lacrimal probe was placed under neck of pterygium and completely dissociated between cornea and head of pterygium.
他说，“无论有或无散光，中低度近视患者的角膜是比较薄的，表面分离器可解决这一问题。
The surface separator solved the problem of thin corneas in patients with low to moderate myopia with or without astigmatism.
方法对47例角膜穿孔伤采用黏弹剂及角膜缘穿刺分离虹膜前粘连。
Methods in 47 cases of penetrating corneal injuries, the viscoelastics was used to dissect the anterior synechia through limbus paracentesis.
目的临床观察不可逆性角膜病变行角膜层间分离术的疗效。
ObjectiveTo observe the clinical outcome of corneal interlamellar dissection for irreversible bullous keratopathy.
目的临床观察不可逆性角膜病变行角膜层间分离术的疗效。
ObjectiveTo observe the clinical outcome of corneal interlamellar dissection for irreversible bullous keratopathy.
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