本文用二步分段盐析法,制备了胎儿淋巴细胞抑制因子(FLIF)。
Fetal lymphocyte inhibitory factors (FLIF) are prepared by means of the successive two-step salting-out procedure using ammonium sulphate.
分段盐析的提纯倍数为(以粗酶提取液为参照)褐藻酸酶13.3 ,琼脂酶8.7和纤维素酶10.9。
The number of purification times of algalase was 13.3 (crude extraction liquid as control ), 8.7 for agaraseand 10.9 for cellulase.
用高速离心、分段盐析、亲和层析、超滤和阴离子交换层析法,从牛胎盘中分离纯化出一种肝细胞生长因子。
A kind of hepatocyte growth factor was purified from cow placenta through high speed centrifugation, salting out, affinity chromatography, ultrafiltration and anion exchange chromatography.
用高速离心、分段盐析、亲和层析、超滤和阴离子交换层析法,从牛胎盘中分离纯化出一种肝细胞生长因子。
A kind of hepatocyte growth factor was purified from cow placenta through high speed centrifugation, salting out, affinity chromatography, ultrafiltration and anion exchange chromatography.
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