目的建立快速检测血清中肾综合征出血热（HFRS）抗体和狂犬抗体的微波免疫荧光方法。
Objective Developing microwave immunofluorescence method for quick detection of hemorrhagic fever with renal syndrome (HFRS) and rabies virus antibody.
方法：使用间接免疫荧光和直接免疫荧光方法对11例RA患者和12例健康人的外周血T淋巴细胞进行了研究。
Methods: T lymphocyte subsets in 11 patients with RA and 12 healthy controls were analyzed by using single and 2 color fluorescence flow cytometry.
采用间接免疫荧光标记方法观察PKC及锚蛋白的细胞内分布情况。
The cellular distribution of PKC and ankyrin was observed by indirect immunofluorescence.
方法：采用间接免疫荧光标记，流式细胞术分析。
Methods Indirect immune fluorescent labeling and flow cytometry were employed.
方法：间接免疫荧光试验等。
方法应用MTT、CFU - L及间接免疫荧光法对18例急性白血病进行观察。
Methods MTT, CFU-L and indirect immune fluorescence were used in 18 patients with acute leukemias.
方法：采用间接免疫荧光流式细胞术，分析K562细胞表面TRAIL分子的表达。
METHODS: The expression of TRAIL on K562 cells was detected by indirect immunofluorescence staining and flow cytometry.
方法骨髓有核细胞免疫荧光标记后用流式细胞术分析。
Methods Bone marrow nuclear cells (BMNCs) were labeled by immunofluorescence and analyzed by flow cytometry.
方法小鼠胎脑细胞体外培养获得神经干细胞，并行免疫荧光检测鉴定。
Methods the cells from the embryonic mouse Brian were primary cultured and identified by immunofluorescence stain.
方法对疑似病例组织标本(皮肤、角膜、脑组织等)运用直接免疫荧光实验法检测狂犬病病毒抗原。
Methods rabies virus antigen was detected for the tissue samples (skin, cornea and brain) from the suspected rabies case by direct fluorescent antibody method (DFA).
目的建立亚马逊利什曼原虫无鞭毛体体外培养方法，并用间接免疫荧光法分析其抗原特异性。
Objective To establish a method for culture of Leishmania amazonensis amastigotes in vitro and identify the antigenic specificity of amastigotes with indirect immunofluorescent assay.
方法应用免疫荧光法检测60例肺癌患者、15例肺良性肿瘤患者和15例正常健康者外周静脉血T细胞亚群和NK细胞活性；
Methods The T subsets and NK cell from peripheral blood of 60 patients with lung cancer, 15 patients with lung benign tumor and 15 healthy people were detected by immunofluorescence.
方法采用细胞免疫荧光技术通过免疫荧光显微镜和激光共聚焦显微镜观察HPK1是否在大鼠脑细胞中存在；
Methods Immunofluorescence technique was used to investigate whether there was HPK1 in hippocampus neuron under the fluorescence microscope and the confocal microscopy.
方法采用三色免疫荧光直接标记法及多参数流式细胞术分析13例多发性骨髓瘤的免疫表型。
Methods Three-colour direct immunofluorescence staining and multiparameter flow cytometry were used for analysis of 13 samples of immunophenotyping of multiple myeloma patients.
方法：本研究采用间接免疫荧光法测定T细胞亚群；
Method:The indirect immunofluorescent assay was used in the present study.
用MTT方法对脾脏的NK杀伤细胞率和淋巴细胞的转化率进行测定，采用免疫荧光法对CD4+、CD8+细胞进行测定。
The killing activity of NK cells and the proliferation rate of spleen cells were tested by MTT assay, and T cell subgroupCD4+ , CD8+ were tested by the indirect immune fluorescent assay.
方法免疫荧光组织化学双重染色，染色结果在激光共聚焦扫描显微镜下观察。
METHODS Immunofluorescence histochemical double staining technique was used and the staining results were observed with confocal laser scanning microscope.
用免疫荧光标记方法检测神经元特异性标记物。
Specific marks of neuron were detected by fluorescent Immunohistochemical stain method.
介绍了应用激光扫描共聚焦显微镜，结合免疫荧光技术研究卵母细胞和早期胚胎细胞骨架的方法。
This study systematically introduced the method of cytoskeleton detection in mouse oocytes and preimplantation embryos by confocal laser scanning microscopy and immunofluorescent staining technique.
方法：荧光金(FG)逆行追踪结合5 HT免疫荧光组织化学染色的双标技术。
METHODS: Fluoro Gold (FG) retrograde tracing combined with serotonin (5 HT) immunofluorescence histochemical staining method was used.
方法SD大鼠单次全脑照射后用免疫荧光组织化学法分别检测早期大脑皮质和海马ca 1区NG2和O4阳性细胞数量。
Methods to determine, by immunofluorescence histochemical method, NG2 and O4-positive cells in the cortex and hippocampal CA1 area of SD rats early after single dose whole-brain irradiation.
方法：用直接免疫荧光法查检鼠肺中的病毒抗原。
Methods: Using direct immunofluorescence assay (DIFA) to detect EHF virus from the lungs of rodents.
方法免疫组织化学染色结合显微图像定量分析和免疫荧光双重染色。
Methods Immunohistochemical staining combined with the micro-image analysis and immunofluorescence histochemical double-staining technique were used.
方法应用间接免疫荧光(IIF)和ELISA方法检测了205例肾脏疾病患者血清中的ANCA及其靶抗原。
Methods ANCA fluorescent patterns and its target antigens in sera of 205 patients with renal diseases were determined by indirect immunofluorescence (IIF) and ELISA.
方法采用间接免疫荧光法检测白血病患者T淋巴细胞亚群、NK细胞百分数，MTT法检测NK细胞杀伤活性。
Methods t lymphoid subgroups and the percentage of NK cells were detected by the use of indirect immunofluorescence method the function of NK cells was detected by MTT.
方法：间接免疫荧光标记法。
方法：采用受体放射配基结合分析、受体-DNA结合分析及免疫荧光技术。
Methods: receptor radioligand binding assay, receptor DNA binding assay and immunofluorescence methods were used.
方法：免疫荧光组织化学双重标记技术。
METHODS: Immunofluorescence histochemical double-staining method was used.
方法免疫组织化学和免疫荧光双标记技术。
Methods Immunohistochemical and double immunofluorescence techniques were used in the study.
方法免疫组织化学和免疫荧光双标记技术。
Methods Immunohistochemical and double immunofluorescence techniques were used in the study.
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