这是最有效的方法，扩增，因为输出设备的最大功率耗散没有输入信号。
This is the least efficient method of amplification, because the output devices are dissipating maximum power with no input signal.
模板扩增和信号放大相结合的有PCR-基因扫描技术、PCR安捷伦芯片实验室技术；
PCR-Gene scan assays and PCR Agilent chip chamber combine the template Amplication and the signal magnification.
模板扩增和杂交以及信号放大相结合的有pcr- ELISA技术、实时荧光pcr技术、生物芯片技术。
PCR-ELISA and real fluorescence PCR associate the template Amplication and hybridization with the signal magnification.
方法用PCR方法扩增il_3信号肽序列和人内皮抑素基因，然后采用重叠延伸pcr拼接的方法将两者拼接和扩增。
METHODS IL_3 signal sequence and human endostatin gene were amplified with PCR and spliced with overlap extension PCR.
这种方法不仅可以直接扩增DNA和RNA，还可以实现对靶核酸的信号放大，灵敏度达到一个拷贝的核酸分子，因此在核酸检测中具有很大的应用价值和潜力。
This method can not only amplify DNA and RNA directly, but also allow the enlargement of signal from target nucleic acids with the sensitivity up to one copy of nucleic acids molecule.
这种方法不仅可以直接扩增DNA和RNA，还可以实现对靶核酸的信号放大，灵敏度达到一个拷贝的核酸分子，因此在核酸检测中具有很大的应用价值和潜力。
This method can not only amplify DNA and RNA directly, but also allow the enlargement of signal from target nucleic acids with the sensitivity up to one copy of nucleic acids molecule.
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