方法：采用全胚胎体外培养技术。
目的：探索及完善精原干细胞的分离、鉴定和体外培养技术；
Objectives:To establish the culture system of spermatogonial stem cells in vitro;
本文就棘球绦虫体外培养技术在棘球蚴病研究中的应用作一综述。
This paper reviews the application of the in vitro cultivation technique of Echinococcus spp.
皮肤细胞的体外培养技术一直是化妆品相关皮肤领域研究的重要手段。
The skin cutaneous-cell culture technologies have been important means in the fi eld of cosmetic and dermatology.
采用小鼠早期胚胎的体外培养技术，研究了久效磷对小鼠着床前胚胎发育的影响。
In this paper, we have studied effects of monocrotophos on the development of preimplantation mouse embryos cultured in a simple defined chemical medium.
应用示踪法和体外培养技术，研究了桑蚕幼虫中肠各部位对亮氨酸和尿嘧啶吸收作用的差异。
The differences between the absorption of leucine and uracil by various parts of mid-gut of silkworm larva were studied, using trace method and culture in vitro technique. During in vitro.
目的探讨沿海地区手部非结核分枝杆菌感染的体外培养技术和要领，为临床确诊，治疗提供直接证据。
Objective to explore the in-vivo culturing technique of mycobacteria from special hand infections in laboratory, and to provide the direct evidence for clinical diagnosis.
方法：采用成纤维细胞体外培养技术，比较正常皮肤成纤维细胞与胎儿皮肤成纤维细胞对羊水的不同反应。
METHODS: a comparison of response to amniotic fluid was performed between the fibroblasts of the skins from fetus and from normal skin with the technique of in vitro culture.
本文对禽类胚胎生物工程技术，包括禽类胚胎体外培养技术、禽类嵌合体技术和禽类基因转移技术的研究进行了综述。
The studies on biological engineering technology of embryo in birds, including the in vitro culture, chimera and transgenic birds are summarized in the essay.
方法应用体外细胞培养技术观察MC3T3 - E1成骨细胞株在不同处理的牙骨质表面的生长状况。
Methods Cell culture technique in vitro had been used to study the growth condition of MC3T3-E1 osteoblast line on the surface of cementum treated with various methods.
哺乳动物早期胚胎的体外培养是胚胎工程技术中一个重要的环节。
The technique of early embryos culture in vitro is an important tache to the mammal embryos engineering technology.
目的研究体外培养的人表皮角质形成细胞的生物学特性，为构建组织工程皮肤提供技术参数。
Objective To study the biological characteristics of in vitro cultured human keratinocytes for providing the technical parameters in constructing tissue engineering skin.
利用ELISA技术检测自身免疫甲状腺病(AITD)患者周围血单个核细胞(PBMC)体外培养产生甲状腺球蛋白抗体(TGA)。
Thyroglobulin antibody (TGA) production in vitro by cultured peripheral blood mononuclear cells (PBMC) from patients with autoimmune thyroid disease (AITD) was measured by ELISA technique.
方法：制备贫血小鼠模型，随机分为给药组和对照组，采用造血祖细胞体外培养等实验血液学技术。
Methods:Anemic models of mice were randomly divided into two groups:administered group and control group. Techniques of hematopoietic progenitor cells culture in vitro, etc were used.
采用微吸管吸取技术，我们体外分离出大鼠的味蕾，将其培养在LAPS芯片上。
Using micropipette, the taste buds were successfully isolated from rat tongue epithelium and then placed on LAPS chip.
目的探索应用离心管培养技术进行体外构建组织工程化关节软骨组织的可行性，并研究其结构和功能。
Objective To explore the feasibility of tissue engineered articular cartilage constructed by the technique of centrifuge tube culture and study its structure and function.
最近，技术已经开发为在体外培养干细胞，提供了前所未有的机遇，为学习和了解人体胚胎学。
Recently, techniques have been developed for the in vitro culture of stem cells, providing unprecedented opportunities for studying and understanding human embryology.
虽然牛卵母细胞体外成熟培养技术已趋于成熟和完善，但卵母细胞成熟机理的研究还有待深入。
Although the technology of In Vitro maturation is more and more maturated, the mechanism of maturation needs further study.
卵母细胞体外成熟培养技术是现代生物技术中的重要内容之一。
Occyte maturation culture technology in vitro is one of the main contents in modern biotechnology.
背景与目的：建立一种稳定的人卵母细胞体外培养成熟技术。
BACKGROUND AIM: To establish a stable in vitro culture technique of human oocytes.
猪卵母细胞的体外成熟培养、成熟卵母细胞的孤雌激活和胚胎培养是胚胎生物工程技术的主要环节。
The culture of porcine oocytes maturation in vitro, parthenogenetic activation of MII porcine oocytes and embryo culture are main technology of embryo biology engineering.
目的探讨建立人皮肤汗腺细胞体外分离及纯化培养的技术。
Objective To investigate the method of isolation and purification of epithelial cells of human eccrine sweat gland in vitro.
本研究旨在探索对虾细胞体外培养的可行技术，为对虾细胞的建系工作奠定基础。
The aim of our studies was to transform shrimp cells and develop techniques for the in vivo culture of shrimp cells.
方法从胚胎大鼠的大脑皮质、海马、纹状体等组织分离神经干细胞，用无血清培养技术在体外进行培养、扩增、传代和诱导分化。
The advantage of serum free and clone culturing technology was performed to isolate, culture, passage and induce neural stem cells from embryonic rat cortex, hippocampus and striatum.
建立完善的精原干细胞体外培养体系，为精原干细胞的体外大量扩增提供技术和方法，为治疗男性不育症等提供相关技术。
Finally, to establish the culture system of spermatogonial stem cells in vitro and to offer the therapy technique for the male sterility.
方法：利用无血清培养和细胞克隆培养技术，从胚胎大鼠海马、纹状体、脊髓等区分离神经干细胞，进行体外扩增培养、传代、贴壁分化观察。
Methods: The advantage of serum free and clone culturing technology was performed to isolate, culture and passage neural stem cells from embryonic rat hippocampus, striatum and spinal cord.
将哮喘病理血清与在体外培养的大鼠肺内皮细胞共同孵育，分别用离心法和显微操纵技术对细胞黏附进行体外研究。
Confluent rat pulmonary endothelial cells were incubated with serum collected from rat blood of normal and asthmatic models, and used for study of endothelial cell adhesion in vitro.
由于在体环境异常复杂，细胞生理或病理现象的研究仍依赖于细胞体外分离和培养技术，因此，构建细胞体外加载实验系统是目前研究细胞的力学响应机制的主要途径。
Because of the staggering complexity of the in vivo environment, studying cellular responses to mechanical stress and strain has relied heavily on the use of in vitro mechanical loading systems.
体外肝细胞培养是生物制药和人工肝支持系统的技术基础。
Cultured cells are the basic element of biologic pharmacy and artificial liver system.
方法利用体外细胞培养技术，通过MTT测定、细胞计数等方法。
Methods To observe the protecfive effecf of TF by measuring MTT metablism and cell numbers and cell culturing in vitro.
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