从健康人外周血提取单核细胞。
方法：人外周血淋巴细胞和植物根尖细胞SCE。
Methods:Sister-chromatid exchange (SCE) was used in human lymphocytes and plant root tip cells.
方法计数晚期肺癌患者和健康人外周血免疫细胞数；
MethodsThe nucleated cell amounts of advanced lung cancer patients and the healthy individuals was counted.
目的：建立从人外周血体外诱导扩增树突状细胞(DC)的方法。
Objective: To set up the method of inducing dendritic cells (DC) from human peripheral blood in vitro.
目的证实人外周血白细胞存在褪黑素受体(MR)及建立检测方法。
Objective to verify whether there exist melatonin receptors (MR) in human peripheral leucocytes and to establish the technique of measuring MR.
目的：研究人外周血淋巴细胞褪黑素受体(MR)的基因与蛋白表达的特点。
AIM: to investigate the melatonin receptor (MR) gene and protein expression in the human peripheral blood lymphocytes.
目的研究氨基酚类化合物对人外周血淋巴细胞及小鼠脾脏细胞DNA的损伤效应。
Objective To study DNA damage induced by aminophenols in human peripheral blood lymphocytes and mouse spleen cells in vitro.
目的：探讨造血基质细胞对人外周血干细胞体外培养扩增及基因转导的促进作用。
Objective: to inquire into the promoting effect of hematopoietic stromal cells on the culture and ex vivo expansion and the gene transduction of human peripheral blood stem cells.
目的比较免疫磁珠法和密度梯度离心法分离纯化人外周血中性粒细胞的可靠性。
Objective To compare the reliability of immunomagnetic beads and density gradient centrifugation in purification and isolation of neutrophilic granulocytes from human peripheral blood.
目的：探讨冻干人胎盘因子(PF)对肿瘤化学治疗(化疗)病人外周血象的影响。
AIM: to evaluate the impact of lyophilized human placenta factor (PF) on the peripheral hemogram of patients treated with chemotherapy.
目的建立脱脂棉柱纯化人外周血T淋巴细胞的方法，并与尼龙棉柱分离法进行比较。
Objective To establish a method to purify T lymphocytes from human peripheral blood mononuclear cells (PBMCs) using cotton column and compare its result with a nylon wool cotton column.
结果小鼠微核试验显示样品均呈阳性，人外周血淋巴细胞微核试验5个样品有4个呈阳性。
The micronucleus test on mice indicated positive; 4 among the 5 samples in the peripheral hemolymph cell micronucleus test on human indicated positive.
方法提取人外周血单个核细胞总RNA，反转录聚合酶链反应（RT-PCR）扩增目的基因；
Methods RNA from human peripheral blood mononuclear cells were extracted and target genes were amplified by RT-PCR.
目的：研究全身炎症反应综合征患者外周血中性白细胞和健康人外周血中性白细胞的基因差异表达。
AIM: to study the difference in gene expression of peripheral blood neutrophils between system inflammatory response syndrome (SIRS) patients and the health volunteers.
内源性ROS用佛波醇酯（PMA）刺激人外周血白细胞产生，并用CL和特异性抗氧化物检测和鉴定；
Endogenetic ROS were produced by phorbol myristate acetate (PMA) stimulating human polymorphonuclear leukocytes (PMN), detected by CL and identified with specific antioxidants.
目的与方法：利用碱性单细胞凝胶电泳(SCGE)技术在体外检测硫芥对人外周血淋巴细胞的损伤情况。
Purpose and Methods: Using the alkaline single cell gel electrophoresis technique (SCGE) detect the DNA damage of lymphocytes in human peripheral blood, which were exposed to Sulfur mustard in vitro.
结果表明，各水样的有机浓集物均可对人外周血淋巴细胞DNA产生不同程度的损伤，存在一定的遗传毒性。
The results showed that DNA damages in human peripheral blood lymphocytes were induced after exposure to the organic extracts from all water samples and the genetic hazard also existed.
目的从人外周血淋巴细胞中克隆出cd 81基因，构建真核表达质粒，并在COS - 7细胞中进行表达。
Aim to clone human CD81 gene from peripheral blood lymphocytes, and construct its eukaryotic expression vector, and then express it in COS-7 cells.
目的检测白细胞介素-2（IL-2）联合IL-12诱导人外周血单个核细胞（PBMC）体外抗肿瘤作用。
Objective To examine the in vitro antitumor activity of normal human peripheral blood mono-nuclear cells(PBMC) induced by interleukin-2(IL-2) and IL-12.
在一定剂量范围内，人外周血淋巴细胞克隆效率与照射剂量呈负相关，HPRT基因突变频率与照射剂量呈正相关。
In the radiation dose range used, the colony efficiency of the lymphocytes was negatively correlated to the radiation dose, while the HPRT mutation frequency is positively correlated.
方法建立人脑胶质瘤和转输人外周血单个核细胞于SCID小鼠体的人肿瘤人淋巴细胞的嵌合模型，并用SEC治疗之。
Methods Human lymphocytes were transplanted to human glioma-bearing SCID mice to establish the chimeric model of human tumor and lymphocytes. SEC was applied for tumor treatment.
方法用SEA刺激人外周血T淋巴细胞，观察细胞的DNA合成、形态、IL -2的产生、细胞表型以及凋亡情况。
MethodsT cells proliferation, IL 2 production, DNA synthesis, cell phenotype and apoptosis induced by the SEA in the first or restimulation were detected.
结论以人外周血单个核细胞基因组dna为模板，扩增人CD 14基因的方法是一种获取CD 14基因简捷、有效的方法。
Conclusion It is a simple and effective method to obtain human CD14 gene from genome DNA of human mononuclear cell in peripheral blood.
探讨脱氧雪腐镰刀菌烯醇（DON）对人外周血单个核细胞参与抗原呈递的低分子量蛋白酶体-2（LMP-2）表达的影响。
To explore the effects of deoxynivalenol (DON) on low molecular weight polypeptide-2 (LMP- 2) expression of human peripheral blood mononuclear cells.
结果提示在人鼻咽癌病人外周血的白细胞DNA中，存在着EBV的基因片段，这对进一步研究EBV在癌变中的作用将是非常重要的。
These results indicated that the presence of EBV genomic fragment in leucocyte DNA of patients from NPC will be important for further study on the role of EBV in carcinogenesis and clinical diagnosis.
本论文在本实验室前期研究基础上，初步建立起了利用彗星试验研究中药单体对人外周血淋巴细胞DNA的损伤与保护作用的方法体系。
In addition, a new method was set up to detect the DNA damage and protection effect of herb compounds on the human peripheral lymphocyte.
应用抗CD3单克隆抗体和基因重组人IL-2共同诱导人外周血单个核细胞，制备抗CD3单克隆抗体活化的杀伤细胞（CD3AK）。
Human CD3AK cells were prepared from peripheral blood mononuclear cells by culturing them in recombinant IL-2 and anti CD3McAb.
作者采用小鼠骨髓瘤细胞系与破伤风类毒素免疫的人外周血淋巴细胞进行融合，对影响人-鼠杂交瘤细胞制备人单抗成功的因素进行了探讨。
The cells from mouse myeloma cell line SP2/0 were fused with human peripheral blood lymphocytes from the donors primed with a conventional dosage of tetanus toxoid (TT).
作者采用小鼠骨髓瘤细胞系与破伤风类毒素免疫的人外周血淋巴细胞进行融合，对影响人-鼠杂交瘤细胞制备人单抗成功的因素进行了探讨。
The cells from mouse myeloma cell line SP2/0 were fused with human peripheral blood lymphocytes from the donors primed with a conventional dosage of tetanus toxoid (TT).
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