一种名字有点象占星书术语的叫Trimastix的单细胞鞭毛虫隐隐出现在人们视野里，它也正好有线粒体基因。
An obscure single-celled flagellate with a name like a character from an Asterix book - Trimastix - also turned out to have mitochondrial genes.
结果用鞭毛蛋白基因作引物的PCR方法具有高灵敏度，其他3种对照结果阴性。
ResultsThe PCR by specific flagellin gene primer has high sensitivity. All of 3 controls were negative.
结论：成功地构建杜氏利什曼原虫无鞭毛体蛋白基因的真核表达重组质粒，并且该基因在NIH3T3细胞中获得了稳定表达。
CONCLUSION: a recombinant eukaryotic expression plasmid of amastin gene of Leishmania Donovani was successfully constructed, and can be expressed stably in the NIH3T3 cells.
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