复苏后分离所得胎肝细胞用锥虫兰染色评估细胞活率，并记录贴壁时间，观察集落生长情况。
The survival rate of thawed cells was evaluated by trypan blue staining, and the adherence time and colony growth were observed.
从转化灶中分离的细胞，失去了在培养中的接触抑制，能在软琼脂培养基中形成集落。
The transformed cells separated from the foci lost their ability of post-confluence inhibition of division, and could grow into colony in soft AGAR medium.
对照组卵丘细胞重构囊胚中9个囊胚中分离出es细胞样集落，5个ES细胞样集落可稳定传代。
In control group es cell -like colonies were harvested from 9 blastocysts, in which 5 es cell-like colonies could be passaged successfully.
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