结果显示不论DNA甲基化与否,所有使用受体细胞限制性内切酶的基因组移植都获得成功。
When genome transplantations were performed using the restriction enzyme minus recipient cells, all the genome transplantations worked regardless of if the DNA was methylated or not.
对胞壁质酶R2基因进行了限制酶切图谱分析。
The restriction map of R2 gene was construed for further use.
RNA病毒的聚合酶基因总体上和宿主密码子使用类型不一致,限制了聚合酶基因的及早和过高表达,但其密码子使用频率对聚合酶的限制是适中的。
RNA polymerase in RNA virus is different from host cells restrict it's high and early expression. The restrict is moderate compared with other virus genes.
They do that in a very special way in that they - restriction enzymes are able to identify a particular sequence of bases in a gene.
限制性内切酶的工作机制很特别,它能识别基因中的一段特定的碱基序列
It takes advantage of the specificity of the restriction enzymes, the fact that we know what the gene sequence that we're looking for, and using this technological process of electrophoresis to identify changes that we predict.
这个方法利用了限制酶特有的性质,我们知道我们在找什么样的基因序列,从而才能利用电泳这个技术流程,来识别出我们预计的情况
If this was a sickle patient, so they had this gene instead it wouldn't get cut and when I went to look for that presence of that gene on this gel, it would appear as one large segment instead of a large one and a smaller one.
而如果是个镰状红细胞贫血的病人,他们的基因是不会被限制酶切割的,因此他们基因的凝胶电泳结果就不会呈现,一大一小的结果,而只有一个大的片段
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