这种技术可在DNA靶标分子的任意位点进行基因敲除、敲入、点突变等操作,无需使用限制性内切酶和连接酶。
The Red mediated recombination can be used to insert, delete or substitute DNA sequences at any desired position on a target molecule without the need for restriction enzymes or DNA ligases.
方法:运用多聚酶链反应-限制性内切酶片段长度多态性技术(PCR - RFLP)检测MTHFR的677位点多态性。
Methods: PCR-RFLP technique was used for detecting the A677V polymorphism site of MTHFR gene.
In this case, this particular restriction enzyme cuts symmetrically like this, but not at the same point.
在我们这种情况下,这个限制性内切酶总是这样对称地切割,但两条链切割的位点不一样
应用推荐