本研究利用聚合酶链式反应技术，成功地克隆了枯草芽孢杆菌缺陷型原噬菌体PBSX阻遏基因及其温度敏感型等位基因。
The repressor gene and its allele-a temperature sensitive mutant have been cloned from the defective prophage PBSX of Bacillus subtilis by means of PCR technique.
每个阻遏物相对于另一个都失去活性，然而，两个基因永远不可能同时得到完全表达。
The repressors were designed to inactivate each other, however, such that the two genes would never be fully expressed at the same time.
Church回忆第一次见到了质粒和基因序列的情形-青霉素酶和胰岛素，阻遏物和干扰素。
Church recalls seeing plasmid and gene sequences for the first time-penicillinase and insulin, the lac repressor and interferon.
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