无血清体外培养树突状细胞的研究 关键词】 树突状细胞；无血清培养液；钙离子载体；外周血 [gap=1784]Key wordsdendritic cell; serum-free medium; calcium ionophore; peripheral blood
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...荧光探针 YT439 酵母膏 YT273 双萤光素酶报告基因检测试剂盒 YT237 OCT-1凝胶迁移突变探针(10μM) YT328 钙离子载体(Ionomycin，5mM) YT812 磷酸化IRS1(Ser302)抗体 YT753 小鼠抗PRMT5抗体 YT429 氨苄青霉素钠溶液(100mg/ml)(细菌培养用) YT535 S-羰基还原酶 ...
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PBMC was isolated by Ficoll desity gradient centrifugation methods first, then the PBMC was stimulated 5 hours by phorbol ester and calcium ionophore. The cells were stained by CD4-FITC and anti IL-17-PE, and CD4+IL-17+T cell was detected by Flow Cytometry.
Ficoll密度梯度离心法分离外周血单个核细胞(PBMC),用佛波酯和钙离子载体刺激5小时后,抗CD4-FITC和抗IL-17-PE标记,流式细胞仪检测CD4+IL-17+T细胞的比例。
参考来源 - 中国汉族人类风湿关节炎易感基因及Th17细胞的研究·2,447,543篇论文数据，部分数据来源于NoteExpress
实验是通过加入允许钙离子从基质进入细胞的离子载体进行的。
It's done here experimentally by adding an ionophore that allows calcium to enter the cells from the medium.
除去离子载体后钙离子浓度恢复正常，NF - AT细胞核中排出。
Up removal of the ionophore calcium level returned to normal and NF-AT is exported from the nucleus.
用新激光衍射法研究了钙离子及离子载体A2 3187对红细胞流变特性的影响。
In order to study effect of extracellular Ca 2+ and ionophore A23187 on erythrocyte deformation, the new ektacytometry is used.
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