间接免疫荧光、ELISA和免疫印迹检测证明，含SO . 7片段的三种重组杆状病毒均在昆虫细胞中表达出相应的活性蛋白。
By IFA, ELISA and Western blot experiments, it was proved all the recombinant baculoviruses containing SO. 7 segment could express biologically active proteins in insect cells.
然后使用重组杆状病毒技术使这些S1亚单位在昆虫细胞和昆虫幼虫中实现了高水平表达。
The modified S1 subunits were abundantly expressed by recombinant autographa californica nuclear polyhedrosis viruses in insect cells and larva.
近年来杆状病毒介导的昆虫表达系统得到了广泛地应用，本课题通过该系统表达CYP2B6重组酶，并进行活性鉴定。
In this study, CYP2B6 recombinant enzyme was obtained using baculovirus expression system and its catalytic activity was measured using bupropion as probe.
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