It was found that the electrostatic repulsion interaction between the polysaccharide core and the enzyme enhanced the enzyme activity and stability. The shell membrane of biomimetic capsule was then fabricated by mimicking the structure and function of cell membrane.
首先,模仿细胞液的成分和功能选用三种具有不同电性的多糖(羧甲基纤维素钠、甲基纤维素和羧甲基壳聚糖钠)作为仿生微囊的液核,经比较可知,与酶分子具有静电排斥作用的多糖更有利于酶构象、活力的维持和酶稳定性的提高;然后,模仿细胞膜的结构和功能构建仿生微囊的壳膜。
参考来源 - 仿生固定化酶制备及其催化特性研究·2,447,543篇论文数据,部分数据来源于NoteExpress
目的观察酶保护剂对提高酶稳定性的作用。
Objective To observe the function of enzymatic protective reagent in improving the enzymes stability.
虫荧光素酶稳定性加强,保存时间长,使用更为方便快捷。
Therefore, the luciferase has enhanced stability and long storage time, and can be used more conveniently and rapidly.
结论糖类可使胰激肽原酶稳定性增强,海藻糖和透明质酸组合物作用最佳。
CONCLUSION the stability of PKase activity can be enhanced by carbohydrates, among which the combination of trehalose and ha was the most effective.
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