新霉素(geneticin, G418)又称遗传霉素,是一种氨基糖苷类抗生素, 是细胞稳定转染最常用的抗性筛选试剂。G418能阻断蛋白质合成,对细菌、酵母、高等植物以及哺乳动物细胞均具有氨基糖苷类毒性,用于真核细胞从转座子Tn5和Tn601稳定转染新霉素(neo)抗性基因的选择和维持。经常用于基因转移、基因敲除、抗性筛选以及转基因动物等方面。
结果:获得一株遗传稳定且黄霉素发酵效价比出发菌株高出170.25%的菌株。
Results:A stable strain was obtained with the titer of flavomycin increased by 170.25% than parent strain.
和制药业的任何人交谈,你都会很快发现遗传学是自发现青霉素以来对制药研究最大的冲击。
Talk to anyone in the drug industry, and you'll soon discover that the science of genetics is the biggest thing to hit drug research since penicillin was discovered.
T1和T2代中潮霉素抗性表现为显性单基因位点的遗传方式,符合孟德尔分离规律,并得到分子鉴定结果的证实。
Mendelian inheritance of hygromycin resistance was showed as single dominant locus and proved by Southern blotting analysis in T1 and T2 generation of all transgenic plants.
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