通过对6种大孔树脂的静态吸附和动态吸附试验，选择HPD600和HPD450两种大孔树脂做柱层析。
Two resins, HPD600 and HPD450, were selected among 6 different macroporous absorption resins after static state and dynamic state absorbance.
目的：选择多核苷酸磷酸化酶快速离子交换层析的最佳条件。
OBJECTIVE:Selection of optimal chromatographic conditions for purification of polynucleotide phosphorylase in fast ion-exchange chromatography.
进一步提供了可选择的额外的纯化步骤，包括溶胞产物过滤、阴离子交换层析、三联体亲和层析和疏水性相互作用层析。
It further provides for optional additional purification steps, including lysate filtration, anion exchange chromatography, triplex affinity chromatography, and hydrophobic interaction chromatography.
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